Ammonium Sulfate Precipitation
Mostrando 1-12 de 464 artigos, teses e dissertações.
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1. Synthesis, Characterization and Exchange Reactions of Layered Double Hydroxides of Copper and Aluminum, Intercalated with Sulfate
Layered double hydroxides (LDHs) of Cu:Al in the molar ratio of 2:1, intercalated with sulfate, sulfate/(Li+, Na+, K+ or NH4+), NO3- and CO32-, were synthesized by co-precipitation with increasing pH. The materials were submitted to exchange reactions using B2SO4 (B = Li+, Na+, K+, NH4+) solutions in an attempt to replace previously intercalated cations or i
J. Braz. Chem. Soc.. Publicado em: 2021-01
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2. ALKALINE PROTEASE PRODUCTION BY Bacillus licheniformis LBA 46 IN A BENCH REACTOR: EFFECT OF TEMPERATURE AND AGITATION
Abstract The production of protease from Bacillus licheniformis LBA 46 was studied in a 6 L reactor using the experimental design tool. The higher protease production was obtained in the exponential phase of growth reaching maximum activity (~3,000 U/mL) after 48 h of fermentation at 30 ºC and 300 rpm in a culture medium made of agroindustrial by-products.
Braz. J. Chem. Eng.. Publicado em: 30/09/2019
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3. Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Abstract Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein f
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/02/2019
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4. Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Abstract Background The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017
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5. Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a
Braz. J. Microbiol.. Publicado em: 2016-09
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6. Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels
OBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats. METHODS: A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate
Clinics. Publicado em: 2015-02
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7. Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues
The aim of this work was to study the production of fibrinolytic protease by Bacillus subtilis I-2 on agricultural residues. Molasses substantially enhanced (63%) protease production (652.32 U/mL) than control (398.64 U/mL). Soybean meal supported maximum protease production (797.28 U/mL), followed by malt extract (770.1 U/mL), cotton cake (761.04 U/mL), gel
Braz. arch. biol. technol.. Publicado em: 2014-10
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8. Production and characterization of surface-active compounds from Gordonia amicalis
Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium
Braz. arch. biol. technol.. Publicado em: 2014-02
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9. Determinação da atividade antitríptica em proteínas de produtos do amendoim isoladas por cromatografia de afinidade
The peanut is an oleaginous plant of high nutritional value, a source of protein and a trypsin inhibitor. Trypsin inhibitors are proteins present in the vegetable kingdom, considered anti-nutritional factors for animals. However, there have been several recent reports about their heterologous and beneficial effects on human health. These important effects ha
Quím. Nova. Publicado em: 2014
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10. Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was
Braz. arch. biol. technol.. Publicado em: 2013-12
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11. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic ac
Braz. arch. biol. technol.. Publicado em: 08/11/2013
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12. Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-
Braz. arch. biol. technol.. Publicado em: 08/11/2013