Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels

AUTOR(ES)

Ribeiro, Joycellane Alline do Nascimento Campos, Serquiz, Alexandre Coellho, Silva, Priscila Fabíola dos Santos, Barbosa, Patrícia Batista Barra Medeiros, Sampaio, Tarcísio Bruno Montenegro, Araújo Junior, Raimundo Fernandes de, Oliveira, Adeliana Silva de, Machado, Richele Janaina Araújo, Maciel, Bruna Leal Lima, Uchôa, Adriana Ferreira, Santos, Elizeu Antunes dos, Morais, Ana Heloneida de Araújo

FONTE

Clinics

DATA DE PUBLICAÇÃO

2015-02

RESUMO

OBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats. METHODS: A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate (30-60%) following precipitation with acetone and was further isolated with Trypsin-Sepharose affinity chromatography. Analyses were conducted to assess the in vivo digestibility, food intake, body weight evolution and cholecystokinin levels in Wistar rats. Histological analyses of organs and biochemical analyses of sera were performed. RESULTS: The trypsin inhibitor from Tamarindus reduced food consumption, thereby reducing weight gain. The in vivo true digestibility was not significantly different between the control and Tamarindus trypsin inhibitor-treated groups. The trypsin inhibitor from Tamarindus did not cause alterations in biochemical parameters or liver, stomach, intestine or pancreas histology. Rats treated with the trypsin inhibitor showed significantly elevated cholecystokinin levels compared with animals receiving casein or water. CONCLUSION: The results indicate that the isolated trypsin inhibitor from Tamarindus reduces weight gain by reducing food consumption, an effect that may be mediated by increased cholecystokinin. Thus, the potential use of this trypsin inhibitor in obesity prevention and/or treatment should be evaluated.

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