Ammonium Sulfate Precipitation
Mostrando 13-24 de 464 artigos, teses e dissertações.
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13. Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtai
Braz. J. Microbiol.. Publicado em: 07/05/2013
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14. Comparison of fibrin adhesives prepared by 3 different methods
INTRODUCTION: Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods. Aim: To compare fibrin tissue adhesives prepared by 3 different methods. METHOD: In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (grou
Int. Arch. Otorhinolaryngol.. Publicado em: 2013-03
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15. Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)
Trypsin inhibitors (TI) have crucial functions in plant defense against pests, and recent studies reveal their diversity in stress response. Disease and herbivore infestations are harmful to plant growth, but the response of TI in Cicer arietinum (L.) in Central India has been less studied. In the present study, we partially characterized the TI from Cicer a
Theor. Exp. Plant Physiol.. Publicado em: 2013
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16. Purificação de bromelina dos resíduos de abacaxi (Ananas comosus L.Merril) por precipitação integrada a sistema bifásico aquoso (PEG/Sulfato de amônio) não convencional / Purification of the residue of bromelain from pineapple (Ananas comosus l.Merril) by precipitation integrated into an aqueous (PEG / ammonium sulfate) non conventional
Bromelina é o nome genérico dado ao conjunto de endopeptidases oriundas dos membros pertencentes à família Bromeliaceae, à qual pertence o abacaxi (Ananas Comosus). Devido aos importantes papéis fisiológicos que desempenham, estas enzimas oferecem um amplo espectro de aplicações terapêuticas comprovadas (ação anti-inflamatória, antitumoral; adju
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/02/2012
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17. Antimycobacterial activity of a Brevibacillus Laterosporus strain isolated from a moroccan soil
The treatment of tuberculosis has become more difficult with the worldwide spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of Mycobacterium tuberculosis. Moreover, the prevalence of human disease caused by atypical mycobacteria has also increased in the past two decades and has further complicated the problem of the treatment
Braz. J. Microbiol.. Publicado em: 2012-12
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18. Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk
Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of
Brazilian Journal of Microbiology. Publicado em: 2012-06
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19. Production of rabbit antibodies against purified Glucose oxidase
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion
Brazilian Archives of Biology and Technology. Publicado em: 2012-02
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20. Desenvolvimento de biossensor contendo polifenoloxidases de sementes de jatobá para análise ambiental / Developmento of biosensor containing polyphenoloxidase jatobá seed for environmental analysis
The polyphenoloxidase (PPO) is responsible for catalyzing the conversion of phenolic compounds to quinones in the presence of oxygen. This enzyme is widely used in biosensor production and selective evaluation of phenol content in industrial effluents. In this work a biosensor modified with enzyme extract containing polyphenoloxidase from Hymenaea stigonocar
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/09/2011
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21. Caracterização do material particulado em Cubatão / CHARACTERIZATION OF PARTICULATE MATTER IN CUBATÃO
This work evaluated the particulate matter (PM) collected at the Environmental Research and Training Center (CEPEMA) located in Cubatão City. Sampling was made between 2009, March and 2010, November for fine, coarse and inhalable aerosol (Mini-Vol) and for the various fractions of PM with a cascade impactor (MOUDI) during eight experimental campaigns. A cli
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/05/2011
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22. Evaluation of operational parameters on the precipitation of endoglucanase and xylanase produced by solid state fermentation of Aspergillus niger
In order to develop cost effective processes for converting biomass into biofuels, it is essential to improve enzyme production yields, stability and specific activity. In this context, the aim of this work was to evaluate the concentration of two enzymes involved in the hydrolysis of biomass, endoglucanase and xylanase, through precipitation. Statistical ex
Brazilian Journal of Chemical Engineering. Publicado em: 2011-03
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23. Hemolytic toxin from the soft coral Sarcophyton trocheliophorum: isolation and physiological characterization
The unifying characteristic of cnidarians is the production of protein and polypeptide toxins. The present study describes the identification of a hemolytic toxin from the soft coral Sarcophyton trocheliophorum. The crude extract was highly cytotoxic (EC50 = 50 ng/mL) against human erythrocytes. It was also tested for hemolytic activity by the blood agar pla
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 05/02/2010
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24. Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis against 10 mM sodium phosphate buffer (pH 7.4), followed by
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2010