Alpha Lactalbumin
Mostrando 1-12 de 40 artigos, teses e dissertações.
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1. Adsorção de proteínas do soro do leite e do glicomacropeptídeo (GMP) na interface hidrofóbica de nanotubos de carbono de paredes múltiplas / Adsorption of whey protein and of the glicomacropeptídeo GMP) in the hydrophobic interface of multiple-walled carbon nanotubes
Neste trabalho, foi estudado o comportamento de adsorção das proteínas α-Lactoalbumina (α-La), β-Lactoglobulina (β-Lg) e o glicomacropeptídeo (GMP) em nanotubos de carbono de paredes múltiplas (MWCNT) na presença e na ausência de eletrólitos da série de Hofmeister, em diferentes valores de pH, visando a compreensão do mecanismo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/02/2012
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2. Efeito das proteinas do soro de leite sobre a colonização de Escherichia coli 0157:H7 na mucosa intestinal de camundongos Balb/C.
The effect of dietary whey protein concentrate on adhesion and colonization of enterohemorrhagic Escherichia coli O157:H7 in small intestine of Balb/C mice was available. Eight groups of the six females each one, had been separate and had randomly and received diet standard (AIN93G) (control group) and modified (AIN93 modified with addition from the fraction
Publicado em: 2008
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3. Concentration of alpha-lactalbumin from cow milk whey through expanded bed adsorption using a hydrophobic resin
The design of novel applications for the use of new high-density adsorbents in the direct recovery of proteins requires analysis of the fluid dynamics and mass transfer characteristics of the column used with the solid-liquid system. In this article we describe the operating parameters related to the use of a high-density, hydrophobic resin (Streamline® Phe
Brazilian Journal of Chemical Engineering. Publicado em: 2005-12
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4. The construction, identification and characterisation of plasmids containing human alpha-lactalbumin cDNA sequences.
We describe the cloning of double-stranded cDNA synthesized from lactating human mammary gland total poly(A)-containing RNA, into the EcoRI site of the plasmid pAT153. Nine recombinants were shown to contain alpha-lactalbumin cDNA sequences as determined by positive hybridisation translation of complementary RNA. Restriction enzyme maps were determined for s
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5. Partial purification of rat alpha-lactalbumin mRNA.
Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiser
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6. Creation and phenotypic analysis of alpha-lactalbumin-deficient mice.
alpha-Lactalbumin is an abundant milk-specific calcium metalloprotein which has an evolutionary relationship to lysozyme. It modifies the substrate specificity of a Golgi galactosyltransferase by forming the lactose synthetase binary complex. Lactose, together with other sugars and diffusible ions, is responsible for the osmotic pressure of milk. To assess t
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7. Rat alpha-lactalbumin has a 17-residue-long COOH-terminal hydrophobic extension as judged by sequence analysis of the cDNA clones.
cDNA for rat alpha-lactalbumin has been cloned in bacterial plasmid, and its sequence has been analyzed. The DNA sequence analysis shows that rat alpha-lactalbumin has 17 extra residues beyond the COOH terminus of the alpha-lactalbumin isolated and sequenced to date from other species. The predicted COOH-terminal sequence is hydrophobic and proline rich and
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8. Inhibition by estradiol of the lactogenic effect of prolactin in primate mammary tissue: reversal by antiestrogens LY 156758 and tamoxifen.
Increasing concentrations of estradiol (E2) ranging from 0.01 to 10 nM were found to inhibit partially but significantly the lactogenic effect of ovine prolactin (oPRL) on alpha-lactalbumin production in primate mammary tissues maintained in organ culture for 9 days. E2 at 10 nM inhibited by 38% (mean) PRL-stimulated alpha-lactalbumin production measured by
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9. Comparison of the nucleotide sequence of cloned human and guinea-pig pre-alpha-lactalbumin cDNA with that of chick pre-lysozyme cDNA suggests evolution from a common ancestral gene.
Nucleotide sequence analyses of essentially full-length copies of human and guinea-pig pre-alpha-lactalbumin cDNAs contained within recombinant plasmids, (i) confirm the presence of 19 amino acid hydrophobic amino terminal peptide extensions encoded within each mRNA; and (ii) provides evidence for the existence of a minor variant of guinea-pig alpha-lactalbu
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10. Functional conversion of the homologous proteins alpha-lactalbumin and lysozyme by exon exchange.
Exons of eukaryotic genes that encode proteins frequently appear to encode structural and/or functional protein units [Gilbert, W. (1978) Nature (London) 271, 501; Blake, C.C.F. (1979) Nature (London) 277, 598]. alpha-Lactalbumin and c-type lysozyme are functionally quite different but structurally highly homologous proteins. Their gene organizations have be
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11. Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice.
The mouse alpha-lactalbumin gene has been replaced with the human gene by two consecutive rounds of gene targeting in hypoxanthine phosphoribosyltransferase (HPRT)-deficient feeder-independent murine embryonic stem (ES) cells. One mouse alpha-lactalbumin allele was first replaced by an HPRT minigene which was in turn replaced by human alpha-lactalbumin. The
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12. Lactation is disrupted by alpha-lactalbumin deficiency and can be restored by human alpha-lactalbumin gene replacement in mice.
Mice carrying either a deletion of the murine alpha-lactalbumin (alpha-lac) gene (null allele) or its replacement by the human alpha-lac gene (humanized allele) have been generated by gene targeting. Homozygous null females are alpha-lac-deficient, produce reduced amounts of thickened milk containing little or no lactose, and cannot sustain their offspring.