Partial purification of rat alpha-lactalbumin mRNA.
AUTOR(ES)
Chakrabartty, P K
RESUMO
Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat alpha-lactalbumin. In the purified mRNA preparation alpha-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342543Documentos Relacionados
- alpha-Lactalbumin mRNA in 4-day lactating rat mammary gland.
- Cytoplasmic delivery of ribozymes leads to efficient reduction in alpha-lactalbumin mRNA levels in C127I mouse cells.
- Lactation is disrupted by alpha-lactalbumin deficiency and can be restored by human alpha-lactalbumin gene replacement in mice.
- Functional conversion of the homologous proteins alpha-lactalbumin and lysozyme by exon exchange.
- The construction, identification and characterisation of plasmids containing human alpha-lactalbumin cDNA sequences.