Affinity Chromatography
Mostrando 13-24 de 2334 artigos, teses e dissertações.
-
13. A broad pH range and processive chitinase from a metagenome library
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene nam
Braz J Med Biol Res. Publicado em: 05/01/2017
-
14. Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles
ABSTRACT Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50 kDa and isoelectr
Braz J Infect Dis. Publicado em: 2016-12
-
15. Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
Abstract Background The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom produc
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 13/10/2016
-
16. Preparation of Two Maleic Acid Sulfonamide Salts and Their Copper(II) Complexes and Antiglaucoma Activity Studies
Two novel proton transfer compounds (HAP)+(SAMAL)- and (HBI)+(SAMAL)-.H2O were obtained from (E)-4-oxo-4-(4-sulfamoylphenylamino)but-2-enoic acid (HSAMAL) and 2-aminopyridine (AP) or 1H-benzimidazole (BI), respectively. Copper(II) complexes of salts and of HSAMAL have also been prepared. They have been characterized by elemental, spectral, thermal analyses,
J. Braz. Chem. Soc.. Publicado em: 2016-10
-
17. STERIC MASS ACTION MODEL FOR LACTOFERRIN ADSORPTION IN CRYOGEL WITH IMMOBILIZED COPPER IONS
Abstract Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel
Braz. J. Chem. Eng.. Publicado em: 2016-03
-
18. Isolation and Characterization of Midgut Lectin From Aedes aegypti (L.) (Diptera: Culicidae)
ABSTRACT The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) such as human three groups A, B, and O (RH+), mouse, rat, guinea-pig, sheep and goat erythrocytes. The highest activity of both crude and isolated mid gu
Braz. arch. biol. technol.. Publicado em: 2015-12
-
19. Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated th
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
-
20. Investigating alpha-globin structural variants: a retrospective review of 135,000 Brazilian individuals
Background: Brazil has a multiethnic population with a high diversity of hemoglobinopathies. While screenings for beta-globin mutations are far more common, alterations affecting alpha-globin genes are usually more silent and less well known. The aim of this study was to describe the results of a screening program for alpha-globin gene mutations in a repres
Rev. Bras. Hematol. Hemoter.. Publicado em: 2015-04
-
21. RECOVERY OF CYCLODEXTRIN GLUCANOTRANSFERASE (CGTase) USING IMMOBILIZED METAL CHELATING AFFINITY CHROMATOGRAPHY
Abstract Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activit
Braz. J. Chem. Eng.. Publicado em: 2015-03
-
22. Nano-Detoxification of Organophosphate Agents by PAMAM Derivatives
For the first time, the adsorption of pesticides such as azinphos-methyl and methamidophos by polyamidoamine (PAMAM) derivatives was studied. Amine groups of PAMAM (G4 and G5) were functionalized with different biomolecules such as folic acid, coumarine, arginine, lysine, and asparagine. Subsequently, the synthesized compounds were used to trap organophospha
J. Braz. Chem. Soc.. Publicado em: 2015-03
-
23. Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels
OBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats. METHODS: A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate
Clinics. Publicado em: 2015-02
-
24. Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli
Human enterokinase (synonym: enteropeptidase, EC 3.4.21.9) light chain (hEKL) gene was designed and artificially synthesized with built-in codon blas towards Escherichia colicodon preference. The synthetic hEKL gene was cloned into prokaryotic expression vector pMAL-s and transferred into the expression strain E. coli BL21 (DE3). Recombinant hEKL protein wit
Braz. arch. biol. technol.. Publicado em: 14/10/2014