Affinity Chromatography
Mostrando 25-36 de 2334 artigos, teses e dissertações.
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25. Determinação da atividade antitríptica em proteínas de produtos do amendoim isoladas por cromatografia de afinidade
The peanut is an oleaginous plant of high nutritional value, a source of protein and a trypsin inhibitor. Trypsin inhibitors are proteins present in the vegetable kingdom, considered anti-nutritional factors for animals. However, there have been several recent reports about their heterologous and beneficial effects on human health. These important effects ha
Quím. Nova. Publicado em: 2014
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26. Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins
In this study, we designed an experiment to predict a potential immunodominant T-cell epitope and evaluate the protectivity of this antigen in immunised mice. The T-cell epitopes of the candidate proteins (EgGST, EgA31, Eg95, EgTrp and P14-3-3) were detected using available web-based databases. The synthesised DNA was subcloned into the pET41a+ vector and ex
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-06
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27. Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography
The aim of the present study was to analyse Taenia solium metacestode antigens that were derived from the unbound fraction of jacalin affinity chromatography and subsequent tert-octylphenoxy poly (oxyethylene) ethanol Triton X-114 (TX-114) partitioning in the diagnosis of human neurocysticercosis (NCC). Immunoassays were designed to detect T. solium-specifi
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-05
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28. EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection
Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world's hot spots on
Braz. J. Microbiol.. Publicado em: 09/04/2013
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29. Production of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection
Cowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Bra
Trop. plant pathol.. Publicado em: 2013-02
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30. Desenvolvimento de vetores não virais para entrega gênica baseados na cadeia leve de dineína Rp3 = : Development of non viral vectors for gene delivery based on dynein light chain Rp3 / Development of non viral vectors for gene delivery based on dynein light chain Rp3
Gene delivery is a promising technique with great medical potential that consists in the introduction of exogenous nucleic acids, and can be applied for gene therapy as well as DNA vaccination. However, its use is still limited by the lack of an ideal delivery vector, which is both safe and efficient. Although much more effective, viral vectors still raise s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/07/2012
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31. Desenvolvimento e aplicação de biorreatores capilares para a triagem de ligantes de Purina Nucleosídeo Fosforilases / Development and application of capillary bioreactors for screening of Purine Nucleoside Phosphorylases ligands
Purine Nucleoside Phosphorylases (PNPs) are key enzymes of the purine salvage pathway, and therefore are considered attractive targets for new drugs search. In this context, the development of effective and selective bioassays for PNP ligands screening is an important task. This work describes the covalent immobilization of human and Schistossoma mansoni PNP
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2012
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32. Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence
When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exoge
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-12
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33. Secretory TAT-peptide-mediated protein transduction of LIF receptor α-chain distal cytoplasmic motifs into human myeloid HL-60 cells
The distal cytoplasmic motifs of leukemia inhibitory factor receptor α-chain (LIFRα-CT3) can independently induce intracellular myeloid differentiation in acute myeloid leukemia (AML) cells by gene transfection; however, there are significant limitations in the potential clinical use of these motifs due to liposome-derived genetic modifications. To produce
Braz J Med Biol Res. Publicado em: 2012-10
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34. A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels of human MK. The His-tagged recombinant scFv was expressed in bacteria, purified by metal affinity chroma
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-03
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35. Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense
Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-02
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36. Comparison of two peptide radiotracers for prostate carcinoma targeting
OBJECTIVES: Scintigraphy is generally not the first choice treatment for prostate cancer, although successful studies using bombesin analog radiopeptides have been performed. Recently, a novel peptide obtained using a phage display library demonstrated an affinity for prostate tumor cells. The aim of this study was to compare the use of a bombesin analog to
Clinics. Publicado em: 2012