20 Hydroxyecdysone
Mostrando 1-12 de 48 artigos, teses e dissertações.
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1. Compared activity of agonist molecules towards ecdysone receptor in insect cell-based screening system
RESUMO: O receptor de ecdisona, naturalmente ativado por hormônios esteroidais, é uma proteína-chave nos processos de muda e reprodução de insetos. A ativação artificial desse receptor por meio de pesticidas específicos induz um processo de ecdise anômala, levando o inseto à morte por dessecação e inanição. Neste trabalho, foi estabelecido um p
Arq. Inst. Biol.. Publicado em: 25/11/2019
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2. Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, i
Braz J Med Biol Res. Publicado em: 11/07/2016
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3. Endocrinologia e controle da vitelogênese em carrapatos = Endocrinology and control of tick vitellogenesis / Endocrinology and control of tick vitellogenesis
Background: Ticks are distributed worldwide, with impacts on human and animal health. The cattle tick Rhipicephalus (Boophilus) microplus is the main parasite that affects livestock in tropical and subtropical regions of the world, causing large economical losses. Tick control methods are based on the application of chemical acaricides, which has resulted in
Publicado em: 2011
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4. Endocrinologia e controle de vitelogênese em carrapatos / Endocrinology and control of tick vitellogenesis
Background: Ticks are distributed worldwide, with impacts on human and animal health. The cattle tick Rhipicephalus (Boophilus) microplus is the main parasite that affects livestock in tropical and subtropical regions of the world, causing large economical losses. Tick control methods are based on the application of chemical acaricides, which has resulted in
Publicado em: 2011
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5. Efeito da adição de colesterol e ecdisona na produção in vitro do baculovírus spodoptera frugiperda MNPV
Dentre as pragas que atacam a cultura do milho no Brasil, destaca-se a Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), conhecida no estádio larval como lagarta-do-cartucho, considerada a praga chave da cultura, alimentando-se da planta em todas as suas fases de crescimento, principalmente dos cartuchos de plantas jovens. Para seu control
Publicado em: 2010
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6. Level and distribution of 20-hydroxyecdysone during Pfaffia glomerata development
The ecdysteroid 20-hydroxyecdysone (20E) is a steroid hormone found in insects and few plants. To analyze its temporal and spatial distribution in Pfaffia glomerata (Amaranthaceae), we selected among 71 accessions, the accession 13 which showed the highest root 20E total quantity (1.48 g/plant root). The 20E was constantly detected in flowers, leaves, stems,
Brazilian Journal of Plant Physiology. Publicado em: 2008-12
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7. Estudo farmacognÃstico e determinaÃÃo da atividade biolÃgica de Vitex gardneriana Schauer (Verbenaceae)
Vitex gardneriana Schauer - Verbenaceae is a shrub encountered in the Brazilian northeastâs ecosystem âcaatingaâ. It is popularly called âjaramataiaâ, âjeremataiaâ, âgirimatoâ, âtamancaâ or âtamanqueiraâ. In the folk medicine, the leaves of V. gardneriana are used as sedative and anti-inflammatory. This work describes pharmacobotanical, phytoche
Publicado em: 2004
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8. 20-Hydroxyecdysone increases levels of transient gene expression in transfected Drosophila cells.
Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, in situ, this increase appears to be due
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9. Establishment of a cell line from lepidopteran wing imaginal discs: Induction of newly synthesized proteins by 20-hydroxyecdysone
A cell line has been developed from the imaginal wing discs of the lepidopteran insect Trichoplusia ni (Hübner). The cells grow in suspension as fluid-filled, multicellular vesicles. The cell line has been designated IAL-TND1. Two-dimensional electrophoresis (isoelectric focusing and sodium dodecyl sulfate/polyacrylamide gels) of [35S]methionine-labeled pro
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10. Prepupal Differentiation of Drosophila Imaginal Discs: Identification of Four Genes Whose Transcripts Accumulate in Response to a Pulse of 20-Hydroxyecdysone
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11. Puffing activities and binding of ecdysteroid to polytene chromosomes of Drosophila melanogaster.
Salivary glands of third instar Drosophila melanogaster larvae were incubated in vitro in the presence of 5 x 10(-6) M 20-hydroxy-ecdysone. Steroid hormone was localized on the polytene chromosomes of the salivary gland by a combination of photoaffinity-labeling and indirect immunofluorescence microscopy. Steroid hormone binding to chromosomal loci and their
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12. Regulatory elements in the first intron contribute to transcriptional regulation of the beta 3 tubulin gene by 20-hydroxyecdysone in Drosophila Kc cells.
We have studied the transcriptional regulation of the beta 3 tubulin gene by the steroid hormone 20-hydroxyecdysone (20-OH-E) in Drosophila Kc cells. A series of hybrid genes with varying tubulin gene lengths driving the bacterial chloramphenicol acetyl transferase (CAT) gene were constructed. The promoter activity was assayed after transient expression in K