Yeast Lysis
Mostrando 1-12 de 104 artigos, teses e dissertações.
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1. Identification of the potential inhibitors of malolactic fermentation in wines
Abstract This exploratory work aims to identify the potential inhibitors of lactic bacterial growth and to propose enological practices to guarantee the occurrence of spontaneous malolactic fermentation (MLF) in wines from traditional and double-pruning management harvests in southeast Brazil. One white wine from a summer harvest and one red wine from a wint
Food Sci. Technol. Publicado em: 26/10/2017
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2. APPLICATION OF RESIDUAL YEAST AS A SOURCE OF REDOX MEDIATORS FOR THE ANAEROBIC DECOLORIZATION OF A MODEL AZO DYE
Abstract This work investigated the anaerobic degradation of the model azo dye Remazol Yellow Gold RNL in batch reactors using discharged residual yeast as the source of redox mediators (RM). Two yeast lysis methods (mechanical lysis and sonication) were tested and optimized to produce a riboflavin-rich yeast lysate. The reactors were operated at 25 ºC for
Braz. J. Chem. Eng.. Publicado em: 2016-12
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3. Antimicrobial activity of Brazilian copaiba oils obtained from different species of the Copaifera genus
The antimicrobial activity of copaiba oils was tested against Gram-positive and Gram-negative bacteria, yeast, and dermatophytes. Oils obtained from Copaifera martii, Copaifera officinalis, and Copaifera reticulata (collected in the state of Acre) were active against Gram-positive species (Staphylococcus aureus, methicillin-resistant S. aureus, Staphylococcu
Memórias do Instituto Oswaldo Cruz. Publicado em: 30/04/2008
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4. Production of beta-1, 3-glucanase from Cellulosimicrobium cellulans 191 for l enzimatic lysis of the yeast Kluyveromyces marxianus var. bulgaricus and the production of beta-galactosidade / Produção de B-1, 3-glucanase de Cellulosimicrobium cellulans 191 para lise enzimatica da levedura Kluyveromyces marxianus var. bulgaricus e obtenção de B-galactosidade
A enzima ß-1,3-glucanase da bactéria Cellulosimicrobium cellulans 191 é capaz de lisar a parede celular de diversas leveduras dentre elas as linhagens de Kluyveromyces sp., produtoras da enzima intracelular ß-galactosidase. Este trabalho visou a produção de ß-1,3-glucanase lítica de Cellulosimicrobium cellulans 191 para obtenção das preparações e
Publicado em: 2008
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5. Beta -1,3 glucanases, proteases e quitinases : produção, purificação e aplicação. / Beta-1,3 glucanases, protease and chitinases : production, purification and application.
The aim of this work was to study the production, purification and application of b-1,3 glucanases, proteases and chitinases. The strain Cellulosimicrobium cellulans 191 was used to study the production of b-1,3 glucanases and chitinases and strains B26 and C. cellulans 191 for the production of proteases, using culture media containing different inductors.
Publicado em: 2006
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6. Produção de [beta]-1,3 glucanases, proteases liticas e quitinases por microrganismos e aplicação na lise de leveduras
The aim of this work was the study of b-1,3 glucanases, proteases and chitinases production by B1, B22, B26, FXX, Oerskovia sp. nO4 and Cellulomonas cartae nº191 strains in culture media containing differents inductors as well as their application on yeast cell lysis. The strains B26 and Cellulomonas cartae nO191 showed highest protease production using the
Publicado em: 2003
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7. Prm1 Prevents Contact-Dependent Lysis of Yeast Mating Pairs†
Membrane fusion requires localized destabilization of two phospholipid bilayers, but unrestrained membrane destabilization could result in lysis. prm1 mutant yeast cells have a defect at the plasma membrane fusion stage of mating that typically results in the accumulation of prezygotes that have fingers of membrane-bound cytoplasm projecting from one cell of
American Society for Microbiology.
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8. An immunological method for detecting gene expression in yeast colonies.
A method for detection of cloned, expressed genes in yeast colonies has been developed. The 70-kilodalton (kDa) mitochondrial outer membrane protein of yeast was used as a model protein. Transformation of a strain deficient in the gene for the 70-kDa protein was performed, and transformed colonies were detected with the antibody decoration technique. This te
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9. The Spheroplast Lysis Assay for Yeast in Microtiter Plate Format
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assa
American Society for Microbiology.
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10. Lysis of Yeast Cell Walls Induced by 2-Deoxyglucose at Their Sites of Glucan Synthesis1
Six sites of 2-deoxyglucose (2DG)–induced lysis on three yeasts (Schizosaccharomyces pombe, Pichia farinosa, and Saccharomyces cerevisiae) coincided with the regions of growth of their glucan layers. Identification of the glucan layer as the site of lysis suggests a mechanism of attack by 2DG or by its derivatives. It is proposed that the glucan layer grow
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11. RNA integrity as a quality indicator during the first steps of RNP purifications : A comparison of yeast lysis methods
BioMed Central.
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12. Lysis of Escherichia coli mutants by lactose.
Growth of Escherichia coli strain MM6-13 (ptsI suc lacI sup), which as a suppressor of the succinate-negative phenotype, was inhibited by lactose. Cells growing in yeast extract-tryptone-sodium chloride medium (LB broth) were lysed upon the addition of lactose. In Casamino Acids-salts medium, lactose inhibited growth, but due to the high K+ content no lysis