The Spheroplast Lysis Assay for Yeast in Microtiter Plate Format
AUTOR(ES)
Ovalle, Rafael
FONTE
American Society for Microbiology
RESUMO
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91499Documentos Relacionados
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