Voltage Regulators
Mostrando 25-32 de 32 artigos, teses e dissertações.
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25. Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases
Rem, Rem2, Rad, and Gem/Kir (RGK) represent a distinct GTPase family with largely unknown physiological functions. We report here that both Rem and Rad bind directly to Ca2+ channel β-subunits (CaVβ) in vivo. No calcium currents are recorded from human embryonic kidney 293 cells coexpressing the L type Ca2+ channel subunits CaV1.2, CaVβ2a, and Rem or Rad,
National Academy of Sciences.
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26. Inward rectification of the IRK1 K+ channel reconstituted in lipid bilayers.
Inwardly rectifying potassium (K+) channels (IRK1) were incorporated into lipid bilayers to test the relative contributions of various mechanisms to inward rectification. IRK1 channels were expressed in Xenopus laevis oocytes and oocyte membrane vesicles containing the channels were fused with lipid bilayers. The major properties of the IRK1 channel were sim
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27. Regulation of Yeast H+-ATPase by Protein Kinases Belonging to a Family Dedicated to Activation of Plasma Membrane Transporters
The regulation of electrical membrane potential is a fundamental property of living cells. This biophysical parameter determines nutrient uptake, intracellular potassium and turgor, uptake of toxic cations, and stress responses. In fungi and plants, an important determinant of membrane potential is the electrogenic proton-pumping ATPase, but the systems that
American Society for Microbiology.
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28. P2Y6 receptor mediates colonic NaCl secretion via differential activation of cAMP-mediated transport
Extracellular nucleotides are important regulators of epithelial ion transport. Here we investigated nucleotide-mediated effects on colonic NaCl secretion and the signal transduction mechanisms involved. Basolateral UDP induced a sustained activation of Cl– secretion, which was completely inhibited by 293B, a specific inhibitor of cAMP-stimulated basolater
American Society for Clinical Investigation.
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29. Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles
Members of the Kv7 voltage-gated K+ channel family are important determinants of cardiac and neuronal membrane excitability. Recently, we and others have shown that Kv7 channels are also crucial regulators of smooth muscle activity. The aim of the present study was to assess the Kv7 expression in different parts of the murine gastrointestinal (GI) tract and
American Physiological Society.
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30. Comparison of the ion channel characteristics of proapoptotic BAX and antiapoptotic BCL-2
The BCL-2 family of proteins is composed of both pro- and antiapoptotic regulators, although its most critical biochemical functions remain uncertain. The structural similarity between the BCL-XL monomer and several ion-pore-forming bacterial toxins has prompted electrophysiologic studies. Both BAX and BCL-2 insert into KCl-loaded vesicles in a pH-dependent
The National Academy of Sciences of the USA.
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31. Regulation of intracerebral arteriolar tone by Kv channels: effects of glucose and PKC
Voltage-gated potassium (Kv) channels in vascular smooth muscle cells (VSMC) are critical regulators of membrane potential and vascular tone. These channels exert a hyperpolarizing influence to counteract the depolarizing effects of intraluminal pressure and vasoconstrictors. However, the contribution of Kv channel activity to the functional regulation of ce
American Physiological Society.
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32. Apparent Absence of a Redox Requirement for Blue Light Activation of Pump Current in Broad Bean Guard Cells1
In guard cells, membrane hyperpolarization in response to a blue light (BL) stimulus is achieved by the activation of a plasma membrane H+-ATPase. Using the patch clamp technique on broad bean (Vicia faba) guard cells we demonstrate that both steady-state- and BL-induced pump currents require ATP and are blocked by vanadate perfused into the guard cell
American Society of Plant Physiologists.