Virus Recombination
Mostrando 1-12 de 1088 artigos, teses e dissertações.
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1. Description of a human Bocavirus recombinant strain in the Americas
Human bocaviruses (HBoV) are mainly associated with respiratory and gastroenteric infections. These viruses belong to the family Parvoviridae, genus Bocaparvovirus and are classified in four subtypes (HBoV1-4). Recombination and point mutation have been described as basis of parvovirus evolution. In this study three viral sequences were obtained from positiv
Mem. Inst. Oswaldo Cruz. Publicado em: 21/10/2019
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2. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were
Braz. J. Microbiol.. Publicado em: 2016-12
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3. Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field
Mem. Inst. Oswaldo Cruz. Publicado em: 17/07/2015
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4. Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversit
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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5. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro l
Braz. J. Microbiol.. Publicado em: 2014-12
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6. First report of recombination in Potato yellow vein virus (PYVV) in Colombia
Potato yellow vein virus (PYVV) is currently one of the most important viruses that infects potatoes in Colombia and other Andean countries, causing losses in the production of tubers ranging from 25% to 50%. This study analyzed the genetic variability of different viral isolates collected in the department of Nariño, Colombia, through bioinformatics analys
Trop. plant pathol.. Publicado em: 2014-06
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7. Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Dengue virulence and fitness are important factors that determine disease outcome. However, dengue virus (DENV) molecular biology and pathogenesis are not completely elucidated. New insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. Unfortunately, instability of flavivirus genomes cloned in E
Mem. Inst. Oswaldo Cruz. Publicado em: 11/10/2013
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8. Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique
O replicon de RNA derivado do genoma de Flavivirus é uma ferramenta valiosa para o estudo de replicação viral independente da montagem e da maturação do virion, além de possuir um grande potencial para expressão de genes heterólogos. Neste estudo nós descrevemos a construção de replicons subgenômicos do vírus da febre amarela utilizando a técni
An. Acad. Bras. Ciênc.. Publicado em: 01/03/2013
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9. CONSTRUÇÃO E MANIPULAÇÃO DE CLONE INFECCIOSO DE UMA AMOSTRA BRASILEIRA DO VÍRUS DA DIARREIA VIRAL BOVINA / CONSTRUCTION AND MANIPULATION OF INFECTIOUS CLONE FROM A BRAZILIAN BOVINE VIRAL DIARRHEA VIRUS ISOLATE
O vírus da diarreia viral bovina (BVDV) é um patógeno de bovinos distribuído mundialmente, associado com importantes perdas econômicas. As maiores perdas devem-se aos problemas reprodutivos causados pela infecção, e pela capacidade do vírus de causar persistência após infecção fetal no terço inicial da gestação. Para entender melhor a biologia
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/03/2012
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10. Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV)
Begomoviruses are whitefly-transmitted single-stranded DNA viruses of great economic importance in the tropics and subtropics. Several begomovirus species have been reported in tomatoes in Brazil, but only a few predominate in the field, for unknown reasons. In this study begomovirus-infected tomato samples were collected in Viçosa, State of Minas Gerais, i
Trop. plant pathol.. Publicado em: 2012-10
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11. Caracterização molecular de recombinantes intersubtipos do vírus da imunodeficiência humana tipo 1(HIV-1) em pacientes provenientes da epidemia do estado de São Paulo. / Molecular characterization of recombinant intersubtipos of human immunodeficiency virus type 1 (HIV-1) in patients from the epidemic in the state of Sao Paulo, Brazil.
The extensive HIV-1 genetic diversity has several implications on the viral adaptive and evolutionary capabilities. This diversity is due to intensive mutational rates derived from error-prone activity of viral reverse transcriptase and RNA genomic instability, generating a highly diverse and heterogeneous viral population named quasispecies. However, even t
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/02/2011
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12. Positive selection, molecular recombination structure and phylogenetic reconstruction of members of the family Tombusviridae: implication in virus taxonomy
A detailed study of putative recombination events and their evolution frequency in the whole genome of the currently known members of the family Tombusviridae, comprising 79 accessions retrieved from the international databases, was carried out by using the RECCO and RDP version 3.31β algorithms. The first program allowed the detection of potential recombin
Genetics and Molecular Biology. Publicado em: 16/09/2011