Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

AUTOR(ES)

Santos, Jefferson José da Silva, Magalhães, Tereza, Silva Junior, José Valter Joaquim, Silva, Andréa Nazaré Monteiro Rangel da, Cordeiro, Marli Tenório, Gil, Laura Helena Vega Gonzales

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

17/07/2015

RESUMO

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

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