Virus Norwalk
Mostrando 1-12 de 136 artigos, teses e dissertações.
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1. Identificação e caracterização molecular de norovírus em surtos de gastroenterites no Estado de São Paulo
Norovírus (NoV), anteriormente chamado Norwalk-like vírus, constituem um importante grupo de patógeno humano associado a surtos de gastroenterites não bacterianas. Levantamentos epidemiológicos de surtos demonstram que as mais importantes vias de transmissão são contatos pessoa-pessoa, água e alimentos contaminados, afetando adultos e crianças. Os N
Brazilian Journal of Microbiology. Publicado em: 2008-12
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2. Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contam
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-03
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3. Detection of human calicivirus (Small round structured virus - SRSV) using polymerase chain reaction (PCR) in oysters from São Paulo beaches, Brazil. / Detecção de Calicivírus Humano (Small Round Structured Virus-SRSV) pela Relação em Cadeia da Polimerase( PCR) em Ostras do Litoral do Estado de São Paulo.
Vírus causadores de gastroenterite, descritos como pequenos vírus de estrutura arredondada (Small Round Structured Viruses-SRSV), foram detectados em extratos de ostras Crassostrea spp., coletadas em regiões distintas do litoral do Estado de São Paulo, utilizando a Reação em Cadeia por Polimerase com transcrição reversa (RT-PCR).Treze lotes de amostr
Publicado em: 1999
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4. Detection of antibody to recombinant Norwalk virus antigen in specimens from outbreaks of gastroenteritis.
Norwalk virus and other small round-structured viruses are commonly associated with outbreaks of gastroenteritis. We used a recently described recombinant-expressed Norwalk virus (rNV) capsid protein in enzyme immunoassays to quantitatively measure immunoglobulin G (IgG) and IgA to Norwalk virus in serum pairs from patients involved in outbreaks of gastroent
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5. Monoclonal antibodies for detection of Norwalk virus antigen in stools.
Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA wit
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6. Inactivation of Norwalk virus in drinking water by chlorine.
Norwalk virus in water was found to be more resistant to chlorine inactivation than poliovirus type 1 (LSc2Ab), human rotavirus (Wa), simian rotavirus (SA11), or f2 bacteriophage. A 3.75 mg/liter dose of chlorine was found to be effective against other viruses but failed to inactivate Norwalk virus. The Norwalk virus inoculum remained infectious for five of
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7. Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adults infected with Norwalk, Snow Mountain, or Hawaii virus.
Subclass-specific antibody responses to the Norwalk virus capsid protein in adults challenged with Norwalk, Snow Mountain, or Hawaii virus were evaluated by solid-phase enzyme immunoassay using recombinant Norwalk virus capsid antigen (rNV). Fourfold or greater serum immunoglobulin G (IgG) antibody responses to rNV were detected in 15 of 20 volunteers challe
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8. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.
Norwalk virus capsid protein was produced by expression of the second and third open reading frames of the Norwalk virus genome, using a cell-free translation system and baculovirus recombinants. Analysis of the expressed products showed that the second open reading frame encodes a protein with an apparent molecular weight of 58,000 (58K protein) and that th
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9. Proteins of Norwalk virus.
The proteins of the Norwalk virus were studied by polyacrylamide gel electrophoresis. Highly purified specifically immunoprecipitated virions appeared to contain a single primary structural protein with a molecular weight of 59,000. In addition, a soluble Norwalk viral protein with a molecular weight of 30,000 was identified in fecal specimens containing Nor
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10. Systemic, Mucosal, and Heterotypic Immune Induction in Mice Inoculated with Venezuelan Equine Encephalitis Replicons Expressing Norwalk Virus-Like Particles
Norwalk-like viruses (NLVs) are a diverse group of single-stranded, nonenveloped, positive-polarity RNA viruses and are the leading cause of epidemic acute gastroenteritis in the United States. In this study, the major capsid gene of Norwalk virus, the prototype NLV, has been cloned and expressed in mammalian cells using a Venezuelan equine encephalitis (VEE
American Society for Microbiology.
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11. Prevalence of antibodies to Norwalk virus in England: detection by enzyme-linked immunosorbent assay using baculovirus-expressed Norwalk virus capsid antigen.
A total of 3,250 serum specimens collected in England in 1991 and 1992 were tested by an indirect enzyme-linked immunosorbent assay for antibody to Norwalk virus using baculovirus-expressed capsid antigen, and 2,382 (73.3%) were positive. The prevalence of Norwalk virus antibody differed regionally. It was lowest (24.6%) in 6- to 11-month-old infants and inc
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12. The isolation and characterization of a Norwalk virus-specific cDNA.
Norwalk virus, an important cause of epidemic, acute, nonbacterial gastroenteritis in adults and children, has eluded adaptation to tissue culture, the development of an animal model, and molecular cloning. In this study, a portion of the Norwalk viral genome encoding an immunoreactive region was cloned from very small quantities of infected stool using sequ