Monoclonal antibodies for detection of Norwalk virus antigen in stools.
AUTOR(ES)
Herrmann, J E
RESUMO
Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228462Documentos Relacionados
- Detection of Escherichia coli enterotoxins in stools.
- Microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools.
- Diagnosis of Norwalk Virus Infection by Indirect Enzyme Immunoassay Detection of Salivary Antibodies to Recombinant Norwalk Virus Antigen
- Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.
- Comparison of Rotazyme and direct electron microscopy for detection of rotavirus in human stools.
