Vicilin
Mostrando 1-12 de 33 artigos, teses e dissertações.
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1. Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine)
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecula
Genetics and Molecular Biology. Publicado em: 20/01/2012
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2. Perfil protéico de sementes de acessos de cacaueiro no desenvolvimento do sabor de chocolate / Proteic profile from different accessions of cocoa seeds on the chocolate flavor development
Typical chocolate flavor is unique, only obtained from fermented, dried and roasted cocoa seeds, and can not be synthesized artificially. The flavor development is influenced by the genetic constitution, post-harvest processing and manufactures. Cotyledons proteins are believed to be the precursors of the chocolate flavor. The aim of the present work was to
Publicado em: 2010
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3. Determinação de motivos de ligação à quitina em vicilinas de Canavalia ensiformis e Vigna unguiculata através de métodos in silico e relação com suas toxicidades para o bruquídeo Callosobruchus maculatus (Coleoptera:Bruchidae)
Chitin is an important structural component of the cellular wall of fungi and exoskeleton of many invertebrate plagues, such as insects and nematodes. In digestory systems of insects it forms a named matrix of peritrophic membrane. One of the most studied interaction models protein-carbohydrate is the model that involves chitin-binding proteins. Among the in
Publicado em: 2009
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4. PurificaÃÃo, caracterizaÃÃo e avaliaÃÃo de atividades biolÃgicas da proteÃna tipo vicilina de Cratylia mollis
Uma proteina sem atividade hemaglutinante ou inibitÃria de tripsina foi isolada de sementes de Cratylia mollis por fracionamento com sulfato de amÃnio e cromatografia em Sephadex G-75 e denominada cratilina. SDS-PAGE na ausÃncia de agente redutor revelou a presenÃa de polipeptÃdeos de Mr 205, 150 e 50 kDa e em condiÃÃes redutoras como peptÃdeos de Mr
Publicado em: 2008
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5. Avaliação da ação bioinseticida de SBTI e vicilina de Erythrina velutina em enzimas digestivas e membrana peritrófica de larvas de Plodia interpunctella (Lepidoptera: Pyralidae)
Plodia interpunctella (Indian meal moth) is a cosmopolitan pest that attacks not only a wide range of stored grain as well other food products. Due to its economic importance several researches have focused in a method with ability to control this pest with few or no damage to the environment. The study of digestive enzymes inhibitors, lectins and chitin-bin
Publicado em: 2007
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6. Expressão heteróloga e caracterização bioquímica da proteína recombinante GmSBP2/S64 da soja (Glycine max) / Heterologous expression and biochemical characterization of GmSBP2/S64 recombinant protein from soybean (Glycine max)
The sucrose binding protein (SBP) belongs to the cupin family of proteins and is structurally related to vicilin-like storage proteins. In this investigation, SBP was expressed in E. coli and large amounts of the protein accumulated in the insoluble fraction as aggregated, denatured protein. The refolding of the purified protein proceeded with a progressive
Publicado em: 2006
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7. Estudo dos mecanismos de defesa de plantas de milho atraves das abordagens de analise proteomica e mapeamento de QTLs
As plantas são capazes de responder e resistir ao ataque de patógenos ativando uma diversidade de estratégias de defesa. A maioria delas exibe uma estratégia geral onde são ativadas respostas bioquímicas de maneira coordenada, incluindo a reprogramação do metabolismo celular, o reforço das barreiras celulares e produção de compostos antimicrobiano
Publicado em: 2005
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8. Identificação e caracterização de proteinas e genes expressos diferencialmente durante o desenvolvimento do embrião zigotico de Araucaria angustifolia
Arauearia angustifolia, the only native conifer in Brazil, covered the States of Paraná (40%), Santa Catarina (31 %) and Rio Grande do Sul (25%). Sparse patches were also found in areas with elevated altitudes, in the States of São Paulo (3%), Minas Gerais and Rio de Janeiro (1 %). The A. angustifolia wood was, for a long time, one of the most important ex
Publicado em: 2002
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9. Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus)
The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Ca
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-02
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10. The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding sequence for preprovicilin.
A cDNA plasmid bank has been constructed using mRNA from developing pea seeds and three cDNAs coding for vicilin polypeptides have been selected. These cDNAs have been sequenced and between them cover the whole of the coding sequence plus part of the 5' and 3' untranslated regions. Comparison with amino acid sequence data from the protein indicates that vici
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11. Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds.
We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S deficiency, transcription of legumin genes inc
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12. Glycoprotein Metabolism in the Cotyledons of Pisum sativum during Development and Germination 1
The glycoprotein nature of legumin and vicilin, the reserve globulins in the cotyledons of Pisum sativum was studied. Legumin from mature seed was found to contain 1% neutral sugars (mannose and glucose) and 0.1% amino sugar (glucosamine), whereas vicilin contained 0.3% neutral sugar (mannose) and 0.2% amino sugar (glucosamine). On the basis of the incorpora