Vap Genes
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Identification of pathogens and virulence profile of Rhodococcus equi and Escherichia coli strains obtained from sand of parks
The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazi
Braz. J. Microbiol.. Publicado em: 20/08/2013
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2. Rhodococcus equi Parte 2: imunologia e profilaxia
Rhodococcus equi é um patógeno ubíquo e habitante da flora intestinal dos equinos de importância na neonatologia equina. Todos os potros são expostos à doença ao nascimento, porém alguns a desenvolvem e outros não. Este artigo revisa características da resposta imune, tanto em adultos competentes quanto em potros suscetíveis ao patógeno. A respos
Ciência Rural. Publicado em: 2011-12
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3. Clonagem, expressão e purificação de proteínas candidatas vacinais de Leptospira interrogans sorovar Copenhageni. Avaliação de atividades imunogênicas e características funcionais. / Cloning, expression and purification of proteins, vaccine candidates from Leptospira interrogans sorovar Copenhageni. Evaluation of immunogenic and functional characteristics.
Leptospirosis is a zoonosis worldwide distributed, caused by bacteria from the genus Leptospira. The development of an efficient vaccine of sub-unities would be of major interest for public health. We proposed to investigate 4 antigens from Leptospira interrogans srv Copenhageni, genes LIC12659, LIC12660, LIC12631 and LIC10868, which were amplified and clone
Publicado em: 2009
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4. CARACTERIZAÇÃO MOLECULAR DE Rhodococcus equi DE POTROS PELA PCR MULTIPLEX DOS GENES DA FAMÍLIA VAP / MOLECULAR CHARACTERIZATION OF Rhodococcus equi FROM FOALS BY MULTIPLEX PCR FOR VAP GENES
O presente estudo tem por objetivo a caracterização molecular de isolados de Rhodococcus equi de eqüinos pela padronização de uma técnica de PCR multiplex para detecção dos genes da família vap (vapA, B, C, D, E, F, G e H). Foram analisadas 180 amostras de Rhodococcus equi de diferentes origens: fezes (112), solo (12) instalações (23) e isolados c
Publicado em: 2008
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5. Construction, phenotypic and gene transcription analysis of an Aggregatibacter actinomycetemcomitans mutant strain in arc B. / Construção, análise do fenótipo e da transcrição gênica de uma amostra mutante de Aggregatibacter actinomycetemcomitans deficiente em arcB.
Aggregatibacter actinomycetemcomitans produces several virulence factors which induce periodontal destruction, but little is known about their regulation. The two components system ArcAB is a genetic transcription regulator that senses oxygen concentrations in the environment. This study aimed to construct an A. actinomycetemcomitans arcB deficient mutant an
Publicado em: 2008
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6. Caracterização de isolados clínicos e ambientais de Rhodococcus equi do Rio Grande do Sul, Brasil utilizando a técnica de reação em cadeia da polimerase multiplex
Rhodococcus equi é uma importante causa de broncopneumonia em potros com menos de 6 meses de idade, sendo responsável por 3% das mortes de eqüinos no mundo. É um microrganismo intracelular capaz de sobreviver e multiplicar no interior de macrófagos. Apresenta 3 níveis de virulência de acordo com os diferentes antígenos expressos em sua superfície. C
Publicado em: 2007
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7. DNA Sequence and Comparison of Virulence Plasmids from Rhodococcus equi ATCC 33701 and 103
The virulence plasmids of the equine virulent strains Rhodococcus equi ATCC 33701 and 103 were sequenced, and their genetic structure was analyzed. p33701 was 80,610 bp in length, and p103 was 1 bp shorter; their sequences were virtually identical. The plasmids contained 64 open reading frames (ORFs), 22 of which were homologous with genes of known function
American Society for Microbiology.
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8. H2O2, Which Causes Macrophage-Related Stress, Triggers Induction of Expression of Virulence-Associated Plasmid Determinants in Rhodococcus equi
The response of the intracellular pathogen Rhodococcus equi to H2O2 treatment, a situation potentially encountered after the oxidative burst of alveolar macrophages, was analyzed. Compared to other bacteria, including Deinococcus radiodurans, R. equi showed exceptionally high resistance to this stress. A proteomic approach showed that four polypeptides prese
American Society for Microbiology.
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9. Virulence Plasmid of Rhodococcus equi Contains Inducible Gene Family Encoding Secreted Proteins
Rhodococcus equi causes severe pyogranulomatous pneumonia in foals. This facultative intracellular pathogen produces similar lesions in immunocompromised humans, particularly in AIDS patients. Virulent strains of R. equi bear a large plasmid that is required for intracellular survival within macrophages and for virulence in foals and mice. Only two plasmid-e
American Society for Microbiology.
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10. Molecular characterization of a genomic region associated with virulence in Dichelobacter nodosus.
The major pathogen implicated in footrot, a highly contagious disease of sheep, is the strict anaerobe Dichelobacter nodosus (formerly Bacteroides nodosus). Sequence analysis of a 2,262-bp segment of the D. nodosus genome which is more prevalent in virulent isolates than in other isolates showed the presence of four open reading frames which appeared to have
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11. The LysR-Type Transcriptional Regulator VirR Is Required for Expression of the Virulence Gene vapA of Rhodococcus equi ATCC 33701
The virulence of the intracellular pathogen Rhodococcus equi in foals is dependent on the presence of an 81-kb virulence plasmid encoding the virulence protein VapA. Expression of this protein is induced by exposure to oxidative stress, high temperatures, and low pHs, which reflect the conditions encountered by R. equi when it enters the host environment. Th
American Society for Microbiology.
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12. Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome.
The recombinant plasmid pJIR318 contains a fragment of the Dichelobacter nodosus genome which is associated with virulence. Sequence analysis of the pJIR318 insert has shown that it contains four vap (virulence-associated protein) genes which are homologous to open reading frames found on the Escherichia coli F plasmid and the Neisseria gonorrhoeae cryptic p