Construction, phenotypic and gene transcription analysis of an Aggregatibacter actinomycetemcomitans mutant strain in arc B. / Construção, análise do fenótipo e da transcrição gênica de uma amostra mutante de Aggregatibacter actinomycetemcomitans deficiente em arcB.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Aggregatibacter actinomycetemcomitans produces several virulence factors which induce periodontal destruction, but little is known about their regulation. The two components system ArcAB is a genetic transcription regulator that senses oxygen concentrations in the environment. This study aimed to construct an A. actinomycetemcomitans arcB deficient mutant and to compare phenotypic carachteristics and gene transcription with the wild type. The growth curves under microaerophilic and anaerobic conditions were similar. There were differences in abilities to adhere and invade epithelial cells, to form biofilm, to adhere to saliva-coated hydroxyapatite and in hydrophobicity between the strains. Gene transcription analysis by real time PCR showed differential expression of apaH, vppA, vapA and omp29. These data indicate that in A. actinomycetemcomitans, ArcB is able to detect redox conditions and is associated with colonization factors of the oral cavity, by regulating transcription of genes associated with virulence.

ASSUNTO(S)

adesão aggregatibacter actinomycetemcomitans pcr em tempo real adhesion gene expression real time pcr biofilmes expressão gênica biofilm aggregatibacter actinomycetemcomitans virulence factors fatores de virulência

ACESSO AO ARTIGO

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