Trypan Blue
Mostrando 25-36 de 239 artigos, teses e dissertações.
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25. Antimycobacterial and cytotoxicity activity of microcystins
Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terra
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 31/03/2015
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26. Cirrose induz apoptose em tecido renal através de estresse oxidativo intracelular
Contexto A falência renal é uma complicação grave e frequente em pacientes com cirrose descompensada. Objetivo Avaliar o estresse oxidativo, o dano ao DNA e alterações na função celular no rim em um modelo animal de cirrose. Métodos A cirrose biliar secundária foi induzida em ratos através da ligadura do duto biliar comum. Foi medido no rim o TBA
Arq. Gastroenterol.. Publicado em: 2015-03
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27. Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo
The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess
Braz J Med Biol Res. Publicado em: 28/03/2014
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28. Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and
Braz J Med Biol Res. Publicado em: 18/03/2014
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29. An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts
PURPOSE: To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. METHODS: Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70ºC. After freezing for a peri
Acta Cir. Bras.. Publicado em: 2013-12
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30. Cytotoxic Effects of Zoledronic Acid on Human Epithelial Cells and Gingival Fibroblasts
A osteonecrose induzida por bisfosfonatos tem sido associada a um efeito citotóxico destes medicamentos sobre as células da mucosa oral. Um estudo recente demonstrou que 5 µM de ácido zoledrônico (AZ), um potente bisfosfonato nitrogenado, foi a maior concentração encontrada na cavidade oral da pacientes em tratamento com este medicamento. Portanto, pa
Braz. Dent. J.. Publicado em: 2013-12
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31. Induced maturation of hepatic progenitor cellsin vitro
Hepatic progenitor cells (HPCs) are a potential cell source for liver cell transplantation but do not function like mature liver cells. We sought an effective and reliable method to induce HPC maturation. An immortalized HP14.5 albumin promoter-driven Gaussian luciferase (ALB-GLuc) cell line was established from HPCs isolated from fetal mouse liver of post c
Braz J Med Biol Res. Publicado em: 16/07/2013
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32. M2-polarized macrophages promote metastatic behavior of Lewis lung carcinoma cells by inducing vascular endothelial growth factor-C expression
OBJECTIVES: Tumor-associated macrophages that generally exhibit an alternatively activated (M2) phenotype have been linked to tumor progression and metastasis. However, the role of M2-polarized macrophages in the growth and metastasis of lung adenocarcinoma remains enigmatic. The aim of this study was to explore the effect of M2 macrophages on the proliferat
Clinics. Publicado em: 2012-08
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33. Avaliação do swim-up e do gradiente descontinuo de densidade na pré-seleção do sexo de espermatozoides na produção de embriões bovinos
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validate
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2012-06
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34. Chlorhexidine: beta-cyclodextrin inhibits yeast growth by extraction of ergosterol
Chlorhexidine (Cx) augmented with beta-cyclodextrin (β-cd) inclusion compounds, termed Cx:β-cd complexes, have been developed for use as antiseptic agents. The aim of this study was to examine the interactions of Cx:β-cd complexes, prepared at different molecular ratios, with sterol and yeast membranes. The Minimal Inhibitory Concentration (MIC) against t
Brazilian Journal of Microbiology. Publicado em: 2012-06
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35. Viability of mesenchymal stem cells during electrospinning
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell i
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-02
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36. Desenvolvimento de protocolo para criopreservação de folículos ovarianos de peixes usando vitrificação / Development of cryopreservation protocol for fish ovarian follicles using vitrification
Cryopreservation of fish embryos has been under investigation for over two decades; however every protocol tested so far has failed. The use of oocytes has more recently been reported as an alternative, nevertheless most of the studies were carried out using a single technique - controlled slow cooling - and success also remains elusive. Vitrification is an
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2012