Trypan Blue
Mostrando 13-24 de 239 artigos, teses e dissertações.
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13. Impact of extracellular alkalinization on the survival of human CD24-/CD44+ breast cancer stem cells associated with cellular metabolic shifts
Cancer stem cells reside in a distinct region within the tumor microenvironment that it is believed to play a fundamental role in regulating stemness, proliferation, survival, and metabolism of cancer cells. This study aimed to analyze the effect of extracellular alkalinization on metabolism and survival of human CD24-/CD44+ breast cancer stem cells (BCSCs).
Braz J Med Biol Res. Publicado em: 20/07/2017
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14. Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro
Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. bra
Braz J Med Biol Res. Publicado em: 10/07/2017
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15. Grandisin induces apoptosis in leukemic K562 cells
Abstract In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both meth
Braz. J. Pharm. Sci.. Publicado em: 20/04/2017
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16. Euphorbia umbellata bark extracts – an in vitro cytotoxic study
ABSTRACT Euphorbia umbellata (Pax) Bruyns, Euphorbiaceae, is commonly used in folk medicine of southern Brazil to treat several kinds of cancer. The latex (part of the plant used for this purpose) is mixed with water and taken as treatment; but this matrix contains toxic potential related to the presence of some phorbol type diterpenes. So the aim of this st
Rev. bras. farmacogn.. Publicado em: 2017-04
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17. Red LED Photobiomodulates the Metabolic Activity of Odontoblast-Like Cells
Resumo Fototerapia tem sido indicada como um tratamento adjuvante para o reparo de tecidos, incluindo o tecido pulpar. Entretanto, não há parâmetros de irradiação definidos, o que representa um grande desafio para o uso clínico da fototerapia. O objetivo deste estudo foi avaliar o efeito da fototerapia com LED vermelho em células MDPC-23 com fenótipo
Braz. Dent. J.. Publicado em: 2016-08
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18. Green Tropical Phytoextracts - Promising Anticancer Alternative
ABSTRACT The anticancer potential of aqueous extracts of tropical plants Ficus deltoidea and Labisia pumila were tested on human prostate carcinoma (DU145) cells. The effects of these extracts on the cells were observed through several tests such as cytotoxicity MTT assay, trypan blue exclusion assay (for cell viability), fluorescence and morphological obser
Braz. arch. biol. technol.. Publicado em: 27/06/2016
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19. Características seminais e de membrana espermática em touros suplementados com tocoferol
RESUMO O trabalho teve como objetivo avaliar a integridade da membrana plasmática e qualidade seminal de touros suplementados com tocoferol. Foram utilizados 16 touros com idade média de 24 meses e peso médio de 462,2kg, em dois tratamentos: grupo controle (GC) e grupo suplementado com tocoferol (GE- 400 UI de tocoferol/ animal/dia) adicionados ao supleme
Rev. bras. saúde prod. anim.. Publicado em: 2016-06
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20. Pravastatina induz parada no ciclo celular e diminuição na produção de VEGF e bFGF em linhagem de Mieloma Multiplo
Resumo O Mieloma Múltiplo é uma neoplasia de linfócitos B da medula óssea, caracterizada por inflamação com uma intensa secreção de fatores de crescimento que promovem o aumento do volume do tumor, sobrevivência celular, migração e invasão. O objetivo deste estudo foi avaliar os efeitos da pravastatina, uma droga usada para reduzir o colesterol,
Braz. J. Biol.. Publicado em: 22/01/2016
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21. Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation
ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis
Braz. arch. biol. technol.. Publicado em: 2015-12
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22. Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation
Apesar de diversos estudos envolvendo regeneração tecidual, incluindo o reparo pulpar induzido por diferentes fontes de luz, dados limitados têm sido reportados a respeito dos efeitos da irradiação com diodos emissores de luz (LED) sobre células-tronco de dentes decíduos esfoliados (SHEDs). O objetivo do presente estudo foi avaliar os efeitos de di
Braz. Dent. J.. Publicado em: 2015-08
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23. Design of signal peptide bombyxin and its effect on secretory expression efficiency and levels of Helicobacter pylori urease subunit B in silkworm cells and larvae
This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus ex
Braz. arch. biol. technol.. Publicado em: 2015-06
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24. Essential oil from Ageratum fastigiatum reduces expression of the pro-inflammatory cytokine tumor necrosis factor-alpha in peripheral blood leukocytes subjected to in vitro stimulation with phorbol myristate acetate
Abstract Ageratum fastigiatum (Gardner) R.M. King & H. Rob., a member of the Asteraceae family popularly known in Brazil as "matapasto", is indicated in folk medicine as anti-inflammatory and analgesic. Despite its popular use, little is known about its potential effect on the parameters involved in an inflammatory response. The objective of this study was t
Rev. bras. farmacogn.. Publicado em: 2015-04