Trypan Blue
Mostrando 1-12 de 239 artigos, teses e dissertações.
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1. Molecular Response of Pulp Fibroblasts after Stimulation with Pulp Capping Materials
Resumo Este estudo avalia in vitro a viabilidade e metabolismo celular, a liberação de óxido nítrico e a produção de duas quimiocinas e uma citocina por fibroblastos de polpa dentária humana em cultura (FPDH) em contato com dois cimentos de ionômero de vidro (Ketac Molar-KM e Vitrebond-VB), Single Bond (SB) e hidróxido de cálcio (Dycal-DY). As cult
Braz. Dent. J.. Publicado em: 2020-06
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2. Use of Inert Gases for the Preservation of Nuclear Blood Cells
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples we
Braz. arch. biol. technol.. Publicado em: 13/06/2019
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3. CELL growth and viability analysis in poli membranes (L-lactic acid-CO-glycolic acid): an in vitro study
RESUMO Objetivo: Esse trabalho avaliou, in vitro, os efeitos biológicos no crescimento e viabilidade celular de fibroblastos em membranas de PLGA com e sem sinvastatina. Materiais e métodos: Foram utilizados dois grupos de membranas reabsorvíveis de polímeros sintéticos: PLGA com sinvastantina e sem sinvastantina, recortadas em formato apropriado para
RGO, Rev. Gaúch. Odontol.. Publicado em: 13/06/2019
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4. Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells
Pituitary adenoma is one of the most common tumors in the neuroendocrine system. This study investigated the effects of long non-coding RNAs (lncRNAs) highly up-regulated in liver cancer (HULC) on rat secreting pituitary adenoma GH3 cell viability, migration, invasion, apoptosis, and hormone secretion, as well as the underlying potential mechanisms. Cell tra
Braz J Med Biol Res. Publicado em: 15/04/2019
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5. Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Abstract Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein f
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/02/2019
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6. Effects of atracurium besylate on corneal endothelium of chickens: in vitro study
RESUMO: Objetivou-se avaliar os efeitos agudos do besilato de atracúrio sobre o endotélio corneano de galinhas. Vinte olhos saudáveis de galinhas foram aleatoriamente separados em dois grupos com 10 olhos cada, sendo G1 o grupo controle e G2 o grupo tratamento. Imediatamente após a excisão dos botões corneoesclerais estes foram colocados em lâminas de
Pesq. Vet. Bras.. Publicado em: 2019-01
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7. Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function
The tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell numbers, cell migration, survival fraction, and cell viabili
Braz J Med Biol Res. Publicado em: 14/11/2018
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8. Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities
Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay,
Braz J Med Biol Res. Publicado em: 06/08/2018
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9. Long non-coding RNA HOTAIR promotes UVB-induced apoptosis and inflammatory injury by up-regulation of PKR in keratinocytes
Excessive exposure to ultraviolet (UV) rays can cause damage of the skin and may induce cancer, immunosuppression, photoaging, and inflammation. The long non-coding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) is involved in multiple human biological processes. However, its role in UVB-induced keratinocyte injury is unclear. This study was performed to
Braz J Med Biol Res. Publicado em: 11/06/2018
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10. miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1
MicroRNAs (miRNAs) have been reported to be associated with heart valve disease, which can be caused by inflammation. This study aimed to investigate the functional impacts of miR-27a on TNF-α-induced inflammatory injury in human mitral valve interstitial cells (hMVICs). hMVICs were subjected to 40 ng/mL TNF-α for 48 h, before which the expressions of miR-
Braz J Med Biol Res. Publicado em: 19/04/2018
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11. 4-Nerolidylcatechol induces autophagy in human glioblastoma cells
ABSTRACT Gliomas account for the majority of primary malignant brain tumors and present invasive behavior into adjacent healthy tissue. While 4-NC had previously shown to induce apoptotic cell death in a melanoma model, for the glioma model described in this paper 4-NC is cytotoxic for the cells with the induction of the autophagic pathway. Trypan blue exclu
Braz. J. Pharm. Sci.. Publicado em: 01/02/2018
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12. Teofilina como agente capacitante do sêmen bovino
RESUMO Objetivou-se avaliar a teofilina como agente capacitante substituto ou associado à heparina sobre a reação acrossômica dos espermatozoides e o desenvolvimento de embriões produzidos in vitro. O experimento foi realizado com quatro touros e três tratamentos, totalizando 12 grupos experimentais. O sêmen dos touros foi avaliado nos tratamentos des
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2017-11