Translatability
Mostrando 1-12 de 37 artigos, teses e dissertações.
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1. Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced
Mem. Inst. Oswaldo Cruz. Publicado em: 15/05/2015
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2. Vida hifenizada: traduzibilidade como exercício de individuação
Esta tese visa delimitar um problema da traduzibilidade, como exercício de individuação, a partir desse conceito situável na obra de Deleuze &Guattari, em particular, em Mille Plateaux. Tal traduzibilidade está localizada pragmaticamente no entre-lugar das línguas portuguesa e castelhana uma tensão de línguas siamesas. A tese se compõe, em primeiro
Publicado em: 2008
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3. Tradutores e tradutoras com e sem formação academica em tradução e suas relações com a teoria e a pratica da tradução poetica
The object of this work is to discuss the strict division between theory and practice of translation, specially concerning the poetic translation, since it is usually considered as the edge of (in)translatability by many theorists of translation and translators, and so because of the issue that involves the relation between theory and practice in the transla
Publicado em: 2004
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4. Translatability of a plant-mRNA strongly influences its accumulation in transgenic plants.
Current knowledge of parameters affecting RNA stability is very restricted in plants. Here we investigated factors which might contribute to the stability of a particular plant messenger RNA. To this end, insertion and deletion mutants were made in two different exons and an intron of the transcribed region of a well characterised patatin gene (pgT5). Mutant
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5. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.
A recombinant vaccinia virus that directs the synthesis of bacteriophage T7 RNA polymerase provides the basis for the expression of genes that are regulated by T7 promoters in mammalian cells. The T7 transcripts, which account for as much as 30% of the total cytoplasmic RNA at 24 hr after infection, are largely uncapped. To improve the translatability of the
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6. A zebrafish nanos-related gene is essential for the development of primordial germ cells
Asymmetrically distributed cytoplasmic determinants collectively termed germ plasm have been shown to play an essential role in the development of primordial germ cells (PGCs). Here, we report the identification of a nanos-like (nanos1) gene, which is expressed in the germ plasm and in the PGCs of the zebrafish. We find that several mechanisms act in concert
Cold Spring Harbor Laboratory Press.
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7. Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.
Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 5' cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, we have determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. We found striking differences in
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8. Cytochalasin releases mRNA from the cytoskeletal framework and inhibits protein synthesis.
Cytochalasin D was shown to be a reversible inhibitor of protein synthesis in HeLa cells. The inhibition was detectable at drug levels typically used to perturb cell structure and increased in a dose-dependent manner. The drug also released mRNA from the cytoskeletal framework in direct proportion to the inhibition of protein synthesis. The released mRNA was
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9. Transcription initiation by RNA polymerase II is inhibited by S-adenosylhomocysteine.
Most eukaryotic mRNAs are blocked at their 5' termini by guanylylation and methylation. These "cap structures" have been shown to play important roles in increasing the stability and translatability of mRNAs. Previous in vitro and in vivo data suggest that these modifications occur extremely early in the synthesis of RNA transcripts by RNA polymerase II. Her
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10. Escherichia coli Endoribonucleases Involved in Cleavage of Bacteriophage T4 mRNAs
The dmd mutant of bacteriophage T4 has a defect in growth because of rapid degradation of late-gene mRNAs, presumably caused by mutant-specific cleavages of RNA. Some such cleavages can occur in an allele-specific manner, depending on the translatability of RNA or the presence of a termination codon. Other cleavages are independent of translation. In the pre
American Society for Microbiology.
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11. The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif
Fragile X syndrome is caused by the absence of protein FMRP, the function of which is still poorly understood. Previous studies have suggested that FMRP may be involved in various aspects of mRNA metabolism, including transport, stability and/or translatability. FMRP was shown to interact with a subset of brain mRNAs as well as with its own mRNA; however, n
Oxford University Press.
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12. Posttranscriptional regulation of hsp70 expression in human cells: effects of heat shock, inhibition of protein synthesis, and adenovirus infection on translation and mRNA stability.
We have examined the posttranscriptional regulation of hsp70 gene expression in two human cell lines, HeLa and 293 cells, which constitutively express high levels of HSP70. HSP70 mRNA translates with high efficiency in both control and heat-shocked cells. Therefore, heat shock is not required for the efficient translation of HSP70 mRNA. Rather, the main effe