Transgenic Plants
Mostrando 13-24 de 1688 artigos, teses e dissertações.
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13. Jaburetox: update on a urease-derived peptide
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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14. Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-09
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15. Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
Abstract Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the
Genet. Mol. Biol.. Publicado em: 27/03/2017
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16. Effect of soybean ureases on seed germination and plant development
Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and
Genet. Mol. Biol.. Publicado em: 02/03/2017
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17. Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of thes
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09
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18. RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
Abstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09
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19. Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease
Abstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-06
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20. The PIN1 family gene PvPIN1 is involved in auxin-dependent root emergence and tillering in switchgrass
Abstract Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, including the regulation of shoot an
Genet. Mol. Biol.. Publicado em: 2016-03
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21. Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from
Braz. arch. biol. technol.. Publicado em: 2015-10
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22. Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor
The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single
Genet. Mol. Biol.. Publicado em: 2014-09
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23. Constitutive overexpression of GmDof17-1, a putative DOF transcription factor from soybean causing growth inhibition in tobacco
The Dof proteins belong to a large family of plant transcription factors that share a single highly conserved zinc finger and play an important role in many physiological processes. To elucidate the function of Dof in soybean, GmDof17-1 was cloned from soybean (Glycine max (L.) Merr); the open reading frame (ORF) of GmDof17-1 is 846 bp and encodes a putative
Sci. agric. (Piracicaba, Braz.). Publicado em: 2014-02
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24. Molecular characteristics, recombinant expression and activity detection of OsGSTL1 from rice
The mRNA of OsGSTL1 was detected in the roots and leaves of rice plants at seedling and tillering stages, and their roots, leaves and panicles at the heading stage. The full-length open reading frame of OsGSTL1 cDNA was 732 bp and encoded a putative polypeptide of 243 amino acids with a calculated molecular mass of 27.30 kDa and a theoretical pI of 5.50. The
Braz. arch. biol. technol.. Publicado em: 2014-02