Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor
AUTOR(ES)
Li, Hui-Liang, Guo, Dong, Peng, Shi-Qing
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
2014-09
RESUMO
The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated by polyethylene glycol, NaCl, and cold treatments, as well as by abscisic acid, jasmonic acid, and ethylene treatment. Analysis of transgenic tobacco plants over-expressing JcR1MYB1 indicates an inportant function for this gene in salt stress.
Documentos Relacionados
- Phenotypic characterization of mutants in vaccinia virus gene G2R, a putative transcription elongation factor.
- Stress molecular physiology of Jatropha curcas seeds.
- The Novel Myb Transcription Factor LCR1 Regulates the CO2-Responsive Gene Cah1, Encoding a Periplasmic Carbonic Anhydrase in Chlamydomonas reinhardtiiW⃞
- A cDNA from a mouse pancreatic beta cell encoding a putative transcription factor of the insulin gene.
- Expression of the CD4 gene requires a Myb transcription factor.