Thaumatin
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Foliar application of Burkholderia sp. strain TNAU-1 leads to activation of defense responses in chilli (Capsicum annuum L.)
Induction of phenolics, peroxidase (PO), polyphenol oxidase (PPO), chitinase and thaumatin-like proteins (TLP) in leaves of chilli (Capsicum annuum L.) in response to foliar application with a biocontrol agent Burkholderia sp. strain TNAU-1 was studied. Chilli plants, when sprayed with Burkholderia sp. strain TNAU-1 showed increase in phenolic content one da
Brazilian Journal of Plant Physiology. Publicado em: 2011
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2. Resistência à ferrugem do cafeeiro: mapeamento genético, físico e análise da expressão gênica em resposta a infecção de H. vastatrix / Coffea leaf rust: genetic, physical mapping and analysis of the gene expression in response to infection the H. vastatrix
The coffee leaf rust it is caused by the biothrofic fungus Hemileia vastatrix is one diseases that more it causes economical damages. The coffee breeding for obtaining for resistance plants it is slow and the moleculars studies of identification for resistance genes to H. vastatrix are still few informative. This study had as objective to obtain information
Publicado em: 2009
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3. Caracterization of a thaumatin-like gene expressed by Moniliophthora Perniciosa fungus, the causative agent of cacao Whiches Broom disease / Caracterização de um gene similar a taumatina expresso pelo fungo causador da Vassoura de bruxa, Moniliophthora perniciosa
Moniliophthora perniciosa is a phytopatogen that has economical importance in Brazil, particularly in the south of Bahia, because this fungus causes the disease in cacao known as Witches Broom disease. This fungus is a hemibiotrofic pathogen that has two distinct stages: a biotrophic (or parasitic) and a necrotrophic (or saprotrophic). The database generated
Publicado em: 2008
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4. Identificação e caracterização de genes potencialmente transferidos horizontalmente no genoma do fitopatogeno C. perniciosa, causador da doença ?vassoura de bruxa? no cacaueiro
Transferência horizontal de genes (HGT) pode ser definida como a transmissão de genes entre diferentes grupos taxonômicos no qual o gene incorporado ao genoma do organismo receptor pode ser mantido ao longo de sucessivas gerações pelo sistema reprodutivo tradicional do receptor. HGT é reconhecida como uma das principais forças atuantes na evolução d
Publicado em: 2005
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5. Thaumatin Production in Aspergillus awamori by Use of Expression Cassettes with Strong Fungal Promoters and High Gene Dosage
Four expression cassettes containing strong fungal promoters, a signal sequence for protein translocation, a KEX protease cleavage site, and a synthetic gene (tha) encoding the sweet protein thaumatin II were used to overexpress this protein in Aspergillus awamori lpr66, a PepA protease-deficient strain. The best expression results were obtained with the gdh
American Society for Microbiology.
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6. Modulation of Aspergillus awamori Thaumatin Secretion by Modification of bipA Gene Expression
Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was
American Society for Microbiology.
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7. A preliminary neutron crystallographic study of thaumatin
Preliminary neutron crystallographic data from the sweet protein thaumatin have been recorded using the LADI-III diffractometer at the Institut Laue Langevin (ILL). The results illustrate the feasibility of a full neutron structural analysis aimed at further understanding the molecular basis of the perception of sweet taste. Such an analysis will exploit the
International Union of Crystallography.
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8. Three-dimensional structure of thaumatin I, an intensely sweet protein.
Thaumatin and monellin are the two sweetest compounds known to man--about 100,000 times sweeter than sugar on a molar basis and 3000 times on a weight basis. These proteins represent a unique class of proteins that are taste-active. We report the three-dimensional structure of thaumatin I at 3.1 A resolution.
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9. Silencing of the Aspergillopepsin B (pepB) Gene of Aspergillus awamori by Antisense RNA Expression or Protease Removal by Gene Disruption Results in a Large Increase in Thaumatin Production
Aspergillopepsin B was identified in culture broths of Aspergillus awamori by in situ detection of its proteolytic activity and by immunodetection with anti-aspergillopepsin B antibodies. Severe thaumatin degradation was observed after in vitro treatment of thaumatin with purified aspergillopepsin B. The pepB gene encoding aspergillopepsin B of A. awamori wa
American Society for Microbiology.
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10. Identification and characterization of a fruit-specific, thaumatin-like protein that accumulates at very high levels in conjunction with the onset of sugar accumulation and berry softening in grapes.
The protein composition of the grape (Vitis vinifera cv Muscat of Alexandria) berry was examined from flowering to ripeness by gel electrophoresis. A protein with an apparent molecular mass of 24 kD, which was one of the most abundant proteins in extracts of mature berries, was purified and identified by amino acid sequence to be a thaumatin-like protein. Co
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11. Characterization of Osmotin 1: A Thaumatin-Like Protein Associated with Osmotic Adaptation in Plant Cells
Cultured tobacco (Nicotiana tabacum var Wisconsin 38) cells adapted to grow under osmotic stress synthesize and accumulate a 26 kilodalton protein (osmotin) which can constitute as much as 12% of total cellular protein. In cells adapted to NaCl, osmotin occurs in two forms: an aqueous soluble form (osmotin-I) and a detergent soluble form (osmotin II) in the
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12. Thaumatin II: a simple marker gene for use in plants.