Subtractive Library
Mostrando 1-12 de 61 artigos, teses e dissertações.
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1. The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear ce
Braz J Med Biol Res. Publicado em: 12/06/2015
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2. Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the micr
Genet. Mol. Biol.. Publicado em: 2014
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3. Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expr
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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4. Seleção e caracterização de peptídeos miméticos a proteínas tumorais no estadiamento clínico-patológico do câncer de próstata
Prostate cancer is the second cause of deaths by tumor in men, surpassed only to lung cancer. Currently, PSA (prostate-specific antigen) is clinical test of choice for PCa detection and treatment. However, the limitations of the PSA test such as large number of false positive results and unnecessary biopsies indicate the need for other means of screening for
Publicado em: 2010
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5. Host respostas transcricionais para vasculares e foliar fungos fitopatogênicos / Host transcriptional responses to vascular and foliar phytopathogenic fungi
O presente trabalho estudou mecanismos genéticos que controlam a resposta de plantas à uma espécie fitopatogênica do gênero Verticillium. V. dahliae (Vd) e V. longisoporum (Vl) são fungos de solo causadores de doenças vasculares de plantas herbáceas e plantas arbóreas, estas duas espécies são responsáveis por perdas anuais que somam bilhões de d
Publicado em: 2009
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6. Identificação de genes expressos durante a fase pré-simbiótica da associação ectomicorrízica entre Hydnangium sp. e Eucalyptus grandis e transformação de fungos ectomicorrízicos / Identification of genes expressed during the pre-symbiotic interaction in the Hydnangium sp.-Eucalyptus grandis ectomycorrhizal association and transformation of ectomycorrhizal fungi
Gene expression in the ectomycorrhizal fungus Hydnangium sp. during the pre-symbiotic interaction was evaluated using an in vitro mycorrhization technique aiming at constructing a suppressive subtractive cDNA library. The fungus was cultivated in the presence of Eucalyptus grandis roots, with no direct physical contact between both organisms. Genes that code
Publicado em: 2008
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7. Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Sup
Genetics and Molecular Biology. Publicado em: 2008
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8. Seleção e caracterização de peptídeos recombinantes ligantes a anticorpos monoclonais reativos a proteínas de Anaplasma marginale
Bovine anaplasmosis is caused by Anaplasma marginale and A. centrale. The most pathogenic and important species for cattle production is A. marginale, and is widely distributed in tropical, subtropical and temperate regions of the world. A. marginale is an intra-erythrocyte rickettsia of susceptible ruminants, biological and mechanically transmitted by ticks
Publicado em: 2008
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9. Análise da expressão gênica no dermatófito Trichophyton rubrum mimetizando a infecção in vitro: pH e diferentes fontes de carbono regulando genes / Analysis of gene expression in the dermatophyte Trichophyton rubrum during the mimetic infection in vitro: pH and carboun sources are regulating genes
Dermatophytes are a group of fungi filamentous that have the ability to invade keratinized substrates, causing dermatophytosis in humans and animals and only penetrate deeper if the host is immunocompromised. Trichophyton rubrum is an anthropophilic and cosmopolitan fungi, the most common agent of superficial mycoses, which uses cell components such as prote
Publicado em: 2008
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10. Genes diferencialmente expressos em Paracoccidioides brasiliensis
Paracoccidioides brasiliensis is a thermally dimorphic fungus causing paracoccidioidomycosis, an endemic disease widespread in Latin America. The dimorphic transition from the mycelia (22 C) to the yeast (36 C) form is induced by a shift from the environmental temperature to that of the mammalian host. Genes that are differentially expressed in phases of P.
Publicado em: 2008
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11. Identificação de genes diferencialmente expressos em tomateiro induzidos por ácido salicílico e por Fusarium oxysporum f. sp. lycopersici.
To identify tomato plant (Lycopersicon esculentum Mill), cv. BRH, genes which answer to plant pathogen Fusarium oxysporum f. sp. lycopersici and salicylic acid, the carrier molecule for activation of responses of plant defense, it was used the suppression subtractive hybridization (SSH) technique, from leaf cDNAs, 24h after salicylic acid, library denominate
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/06/2007
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12. Molecular comparison of pathogenic isolates of the infectious bursal disease virus in Minas Gerais State and construction of a subtractive library / Comparação molecular de isolados patogênicos do vírus da doença infecciosa bursal do estado de Minas Gerais e construção de uma biblioteca subtrativa
A doença infecciosa bursal (IBD) tem sido, há muitos anos, uma grande preocupação para a indústria avícola, especialmente na década passada devido a emergência de cepas virais hipervirulentas. Mutações no gene responsável pela codificação da proteína viral VP2 de linhagens patogênicas e a má utilização de vacinas atenuadas por poucas passag
Publicado em: 2007