Subtractive Library
Mostrando 13-24 de 61 artigos, teses e dissertações.
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13. A capacidade de infecção do dermatófito Trichophyton rubrum está correlacionada com a sinalização do pH extracelular / The infection capacity of the dermatophyte Trichophyton rubrum is correlated with extracellular pH signaling
Dermatofitoses são comumente causadas por fungos que parasitam pele e unha de humanos, cuja propagação depende do contato entre os hospedeiros infectados e não infectados. Muitos fatores contribuem para a patogenicidade dos dermatófitos, dentre eles, a capacidade de se instalar no ambiente ácido da pele se reveste de importância. Sendo assim, para ser
Publicado em: 2007
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14. Identificação e caracterização de biomarcadores teciduais e sorológicos no câncer de mama por Phage Display
CHAPTER II: Breast cancer is one of the main causes of death among women, as there is no primary prevention. Early detection is the main objective aiming decrease mortality and increase survival. We used phage display technology to isolate ligand peptides to breast cancer tissues in order to select potential biomarkers for the improvement of diagnosis and tr
Publicado em: 2007
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15. Identificação de genes potencialmente envolvidos na interação tomateiro - Potyvirus / Identification of genes potentially involved in the interaction tomato - Potyvirus
During co-evolution between virus and host, a complex interaction has been developed involving several mechanisms of pathogen attack and host defense. Host defense responses cause up- and downward shifts in gene expression. However, the effects of viral infection in the hosts gene expression profile are still poorly understood. With the objective of identify
Publicado em: 2006
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16. Witches broom disease of cocoa : genome organization, genetic variability and anlysis of the identity and expression of pathogenicity genes of the fungal pathogen Crinipellis perniciosa / Vassoura-de-bruxa : organização genomica, variabilidade de isolados e analise da identidade e expressão de genes ligados a patogenicidade do fungo Crinipellis perniciosa
Witches broom disease of cocoa is a complex pathosystem that has evaded an efficient control program in the cacao-producing region of Bahia, Brazil. The main goal of this work was to acquire a better understanding of the C. perniciosa-Cacao interaction by providing new data regarding: 1) the size and organization of the fungal genome through molecular karyot
Publicado em: 2006
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17. Cloning and characterization of glucose-regulated genes in human pancreatic islets / "Clonagem e caracterização de genes regulados por glicose em ilhotas pancreáticas humanas"
Type 1 Diabetes mellitus (T1DM) is caused by autoimmune destruction of the insulin-producing pancreatic islet b-cells. Treatment is generally approached by daily subcutaneous injections of exogenous insulin. Nowadays, pancreatic islet transplantation is considered as an effective alternative treatment to insulin therapy. However, in order to reach insulin-in
Publicado em: 2002
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18. Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.
The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to undifferentiated NEC14 cells was constructed using oligo(dT)30-Latex and polymerase chain reaction (PCR). The method was designed to improve the efficiency of subtraction and
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19. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs with
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20. Identification of genes up-regulated in dedifferentiating Nicotania glauca pith tissue, using an improved method for constructing a subtractive cDNA library.
Pith explants of Nicotiana glauca grown in vitro in synthetic medium supplemented with 2,4 dichlorophenoxyacetic acid (2, 4 D), are induced to dedifferentiate. Treatment with actinomycin D within the first 4-8 h of culture (but not later) is lethal and the explants die, implying a requirement for de novo transcription. The genes expressed during the initial
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21. Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.
We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fi
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22. Differential cDNA cloning by enzymatic degrading subtraction (EDS).
We describe a new method, called enzymatic degrading subtraction (EDS), for the construction of subtractive libraries from PCR amplified cDNA. The novel features of this method are that i) the tester DNA is blocked by thionucleotide incorporation; ii) the rate of hybridization is accelerated by phenol-emulsion reassociation; and iii) the driver cDNA and hybr
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23. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments
In the murine thymus, the stroma forms microenvironments that control different steps in T cell development. To study the architecture of such microenvironments and more particularly the nature of communicative signals in lympho–stromal interaction during T cell development, we have employed the phage antibody display technology, with the specific aim of i
The National Academy of Sciences of the USA.
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24. Phenol emulsion-enhanced DNA-driven subtractive cDNA cloning: isolation of low-abundance monkey cortex-specific mRNAs.
To isolate cDNA clones of low-abundance mRNAs expressed in monkey cerebral cortex but absent from cerebellum, we developed an improved subtractive cDNA cloning procedure that requires only modest quantities of mRNA. Plasmid DNA from a monkey cerebellum cDNA library was hybridized in large excess to radiolabeled monkey cortex cDNA in a phenol emulsion-enhance