Substrate Specificity
Mostrando 13-24 de 1966 artigos, teses e dissertações.
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13. Epidemiologia molecular de Escherichia coli e Klebsiella spp produtoras de beta-lactamase de espectro ampliado
The extended-spectrum beta-lactamases (ESBL) represent an important mechanism of resistance to beta-lactam antibiotics. Such enzymes have spread among members of the Enterobacteriaceae family, which are responsible for infections related to health care, particularly nosocomial infections. Objectives: (a) To determine the prevalence of ESBL producing Escheric
Publicado em: 2009
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14. Characterization of the type 2A phosphatase regulatory protein, TIPRL and alpha4 / Caracterização das proteinas TIPRL e alfa4, reguladores de fosfatases 2A
Cells respond constantly to a variety of stimuli, which are interpreted and integrated through signaling networks, giving rise to biological responses. Defects in this circuitry are a cause of many diseases, including cancer. Protein phosphatases are enzymes which remove phosphate groups from kinase substrates, relying mainly on regulatory subunits for their
Publicado em: 2009
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15. Flavins promote changes in the extracellular matrix, signal transduction, antioxidant enzymes and metalloproteinases during osteoblast differentiation / Flavinas promovem mudanças na matriz extracelular, vias de transdução de sinal, enzimas antioxidantes e metaloproteinases durante a diferenciação de osteoblastos
Riboflavin (Rb-Vitamin B2) is the precursor of essential flavocoenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). These coenzymes participate in numerous enzymatic processes dependent on electron transfer reactions that occur in energyproducing, biosynthetic, and detoxifying and electron-scavenging pathways. Increase dietary ribofla
Publicado em: 2009
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16. Atividade de fosfatases em duas cepas de Trypanosoma cruzi
Trypanosoma cruzi, the etiological agent of Chagas`disease, displays a remarkably high degree of both, structural and functional intraspecific heterogeneity, which could modulate its pathogenicity, survival and adaptability. In higher eukaryotes, phosphorylation and dephosphorylation are fundamental pathways that regulate a wide variety of cellular events. T
Publicado em: 2008
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17. Partial biochemistry characterization and obtention of low molecular weight acid phosphatase from osteoblasts cultures / Perfil da proteína tirosina fosfatase de baixo peso molecular em células osteoblásticas
Events as phosphorilation and desphosphorilation are experienced in cell growth and differentiation processes. Tyrosine phosphatases proteins are involved in these processes. These enzymes are found in animals, plants and occur in multiple forms, differing in relative molecular weight, substrate specificity and sensitivity to inhibitors. The enzymes that hav
Publicado em: 2008
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18. The role of autolysis loop in determining the specificity of coagulation proteases
We recently demonstrated that the substitution of the autolysis loop (residues 143 to 154 in the chymotrypsin numbering system) of activated protein C (APC) with the corresponding loop of factor Xa (fXa) renders the APC mutant (APC/fX143-154) susceptible to inhibition by antithrombin (AT) in the presence of pentasaccharide. Our recent results further indicat
Brazilian Journal of Medical and Biological Research. Publicado em: 22/06/2007
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19. Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner
We examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s) from S. japonicum and S. mansoni cleaved human hemoglobin. In addition, aspartic protease activities from S. japonicum cleav
Memórias do Instituto Oswaldo Cruz. Publicado em: 2007-02
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20. Sensores eletroquímicos: considerações sobre mecanismos de funcionamento e aplicações no monitoramento de espécies químicas em ambientes microscópicos
Electrochemical sensors have attracted considerable attention in recent years because they provide data about the chemical state of our surroundings and the dynamics of the chemical transformations in the form a spatially resolved image. Particular interest has been directed to measurements in restricted-volume samples as new technologies enable the fabricat
Química Nova. Publicado em: 2006-12
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21. Nucleotide-sugar transporters: structure, function and roles in vivo
The glycosylation of glycoconjugates and the biosynthesis of polysaccharides depend on nucleotide-sugars which are the substrates for glycosyltransferases. A large proportion of these enzymes are located within the lumen of the Golgi apparatus as well as the endoplasmic reticulum, while many of the nucleotide-sugars are synthesized in the cytosol. Thus, nucl
Brazilian Journal of Medical and Biological Research. Publicado em: 2006-09
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22. Estudo das bases moleculares da especificidade pelo substrato de uma beta-glicosidase (AF052729) de Spodoptera frugiperda / Study of the molecular basis of substrate specificity in a Spodoptera frugiperda beta-glycosidase (AF052729)
The Mr 50,000 Spodoptera frugiperda beta-glycosidase (Sfgli50) has active site amino acid residues that interact non-covalently with the substrate. These non-covalent interactions determine the enzymes specificity towards its substrates. This work aims to study the role of these amino acids and its interactions with the substrate in the specificity of Sfgl
Publicado em: 2006
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23. Purification, kinetics and physical-chemistry characterization of periplaneta americana, Tenebrio molitor and Diatraca saccharalis digestive chymotrypsin / Purificação, caracterização físico-químico e cinética das quimotripsinas digestivas de Periplaneta americana, Tenebrio molitor e Diatraea saccharalis.
Chymotrypsins (EC 3.4.21.1) are serine proteases with 245 amino acids disposed in two double ?-barrel domains, being the active site locate between these two domains. The specificity study of endopeptidases was facilitated by the substrate binding site concept that is the active site of these enzymes. However there is not much information about the subsites
Publicado em: 2006
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24. Desenvolvimento de aplicações tecnológicas da metodologia de phage display no diagnóstico do câncer de próstata
Prostate cancer is the second cause of death in men by tumor, surpassed only by lung cancer. Its occurrence is estimated to be 51 to 100,000 cases per year. The increasing incidence levels observed may be partially explained by the evolution of diagnostic methods, quality of information systems and longer life expectancy. The identification of new cancer spe
Publicado em: 2006