Spin Label
Mostrando 25-36 de 162 artigos, teses e dissertações.
-
25. A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes
A technique is described for the measurement of lateral diffusion of any spin-labeled molecule in planar phospholipid multilayers, using low probe concentrations and a photochemical reaction to generate the initial concentration gradient. Features of the technique include simplicity of data analysis, versatility, and applicability over a wide range of motion
-
26. Electron-electron double resonance and saturation-recovery studies of nitroxide electron and nuclear spin-lattice relaxation times and Heisenberg exchange rates: lateral diffusion in dimyristoyl phosphatidylcholine.
Lateral diffusion constants of the stearic acid nitroxide radical spin label 2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolindinyl oxide in dispersions of dimyristoyl phosphatidylcholine have been measured. Electron-electron double resonance methods were used to determine the product of the bimolecular collision frequency and T1e, the electron spin-l
-
27. Spin-labeled polyribonucleotides.
Poly (U), poly (C) and poly (A) were spin labeled with N-(2,2,5,5-tetramethyl-3-carbonylpyrroline-1-oxyl)-imidazole. This spin label interacts selectively with 2' OH ribose groups of polynucleotides and does not modify the nucleic acid bases. The extent of spin labeling is not dependent upon the nature of the base and is entirely determined by rigidity of th
-
28. A spin label that binds to myosin heads in muscle fibers with its principal axis parallel to the fiber axis.
We have used an indane-dione spin label (2-[-oxyl-2,2,5,5-tetramethyl-3-pyrrolin-3-yl)methenyl]in dane-1,3-dione), designated InVSL, to study the orientation of myosin heads in bundles of chemically skinned rabbit psoas muscle fibers, with electron paramagnetic resonance (EPR) spectroscopy. After reversible preblocking with 5,5'-dithiobis(2-nitro-benzoic aci
-
29. Membrane location of apocytochrome c and cytochrome c determined from lipid-protein spin exchange interactions by continuous wave saturation electron spin resonance.
Apocytochrome c derived from horse heart cytochrome c was spin-labeled on the cysteine residue at position 14 or 17 in the N-terminal region of the primary sequence, and cytochrome c from yeast was spin-labeled on the single cysteine residue at sequence position 102 in the C-terminal region. The spin-labeled apocytochrome c and cytochrome c were bound to flu
-
30. Temperature-Induced Protein Conformational Changes in Barley Root Plasma Membrane-Enriched Microsomes: I. Effect of Temperature on Membrane Protein and Lipid Mobility
A protein spin label and lipid spin probes were used to study the temperature-dependent motion of protein and lipid, respectively, in barley (Hordeum vulgare L. cv Conquest) root plasma membrane-enriched microsomes. Using membranes from seedlings grown at 20°C, the temperature-dependence of the relative motion of membrane surface spin probes and a spin labe
-
31. Neisseria gonorrhoeae Membrane Microenvironment Studied by Spin-Label Electron Spin Resonance: Comparison of Colony Types
-
32. Reactivity of reverse transcriptase toward (s4U,U)n copolymers and spin-labeled nucleic acid lattices.
Spin-labeled copolymers of 4-thiouridine and uridine (ls4U,U)n] that contain various amounts of spin label (l) were synthesized by either (i) chemical alkylation of the 4-thiouridine-uridine copolymers (s4U,U)n prepared by copolymerizing 4-thiouridine 5'-diphosphate (s4UDP) and UDP or (ii) copolymerization of spin-labeled s4UDP with UDP using polynucleotide
-
33. Conformational change and cooperativity in actin filaments free of tropomyosin.
The decrease in amplitude of the electron spin resonance spectrum of the cysteine-bound spin-label, 3-(maleimidomethyl)-2,2,5,5-tetramethyl-1-pyrrolidinoxyl, brought about by the magnetic interaction with tightly bound manganous ion, was used as a probe of conformational change in actin on binding myosin. The magnitude of this "spin--spin" interaction first
-
34. Fluorescence quenching and electron spin resonance study of percolation in a two-phase lipid bilayer containing bacteriorhodopsin.
The effect of bacteriorhodopsin (BR) on the percolation properties of dimyristoylphosphatidylcholine/distearoylphosphatidylcholine bilayers was examined by studying the quenching of a lipid-bound fluorophore by a lipid-bound quencher, and by spin-spin interactions of a nitroxide-labeled lipid using electron spin resonance (ESR). At the low concentrations of
-
35. Electron Spin Resonance Studies of Erythrocytes from Patients with Myotonic Muscular Dystrophy
Electron magnetic resonance experiments have demonstrated that spin-labeled myotonic erythrocyte membranes have spectra that are recognizably different from those of normal erythrocytes. The spin label incorporated in the erythrocyte membranes of patients having myotonic muscular dystrophy is apparently located in a less polar and somewhat more fluid region
-
36. Immune Lysis of Liposomes and Erythrocyte Ghosts Loaded with Spin Label*
It is shown that the paramagnetic resonance of spin labels offers a convenient technique for monitoring the complement-mediated immune lysis of erythrocyte ghosts, and sensitized phospholipid liposomes. The ghosts or liposomes are loaded with a concentrated solution of a water-soluble, membrane-impermeable spin label, so that there is a strong exchange broad