Immune Lysis of Liposomes and Erythrocyte Ghosts Loaded with Spin Label*
AUTOR(ES)
Humphries, Gillian Kitch
RESUMO
It is shown that the paramagnetic resonance of spin labels offers a convenient technique for monitoring the complement-mediated immune lysis of erythrocyte ghosts, and sensitized phospholipid liposomes. The ghosts or liposomes are loaded with a concentrated solution of a water-soluble, membrane-impermeable spin label, so that there is a strong exchange broadening of the paramagnetic resonance signal due to labels enclosed within the liposomes or ghosts. Complement-mediated lysis releases the labels into a dilute solution, where a relatively sharp and intense paramagnetic resonance signal is detected.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388304Documentos Relacionados
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