Simple Sequence Repeats Ssr
Mostrando 25-36 de 40 artigos, teses e dissertações.
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25. Caracterização molecular de videiras de Santa Catarina por marcadores microssatélites
O objetivo deste trabalho foi caracterizar a diversidade de videiras em Santa Catarina, por meio de marcadores moleculares microssatélites ("simple sequence repeats" - SSR). Amostras de DNA foram coletadas a partir de folhas e ramos de acessos de coleções de germoplasma públicas e privadas, nos municípios de Urussanga, Nova Trento, Rodeio, São Joaquim,
Pesquisa Agropecuária Brasileira. Publicado em: 2009-05
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26. anÃlise in silico deEST-SSR em phaseolus vulgaris e Glycine max e transferibilidade de marcadores para Vigna unguiculata
Expressed Sequence Tags (EST) database is a valuable source of information for molecular markers development. In the present work, simple sequence repeats (SSR) have been detected in Phaseolus vulgaris and Glycine max ESTs, aiming the development of ESTSSR markers for cowpea (Vigna unguiculata) improvement. A total of 10,880 common bean ESTs and 116,965 soy
Publicado em: 2008
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27. Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley
Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and
Genetics and Molecular Biology. Publicado em: 2008
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28. CaracterizaÃÃo molecular (PCR) e infecÃÃo de Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus / Molecular Characterization (PCR) and infection Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus
The Metarhizium anisopliae var. acridum and M. anisopliae var. anisopliae strains were analysed for the pathogenicity to the fly Zaprionus indianus, using the concentrations 104, 105, 106, 107, 108 conidia/mL, considering the percentage of adultsâ emergency. In agreement with the used methodology, it was verified that both fungi strains presented action aga
Publicado em: 2006
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29. In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with
Genetics and Molecular Biology. Publicado em: 2005
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30. CaracterizaÃÃo molecular de espÃcies de Metarhizium e patogenicidade sobre Diatraea saccharalis / Molecular characterization of Metarhizium and pathogenicity on Diatraea saccharalis
Fifteen Metarhizium strains isolated from different areas and hosts were analysed upon genetic characteristics and 7 strains upon the pathogenicity to Diatraea saccharalis. The ITS (Internal Transcrided Spacer) molecular markers of rDNA, Intron splice site primer, RAPD and Microsatelite (SSR-Simple Sequence Repeats) were used to evaluate the genetic diversit
Publicado em: 2005
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31. Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
In order to compare their relative efficiencies as markers and to find the most suitable marker for maize diversity studies we evaluated 18 inbred tropical maize lines using a number of different loci as markers. The loci used were: 774 amplified fragment length polymorphisms (AFLPs); 262 random amplified polymorphic DNAs (RAPDs); 185 restriction fragment le
Genetics and Molecular Biology. Publicado em: 2004
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32. Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty hig
Genetics and Molecular Biology. Publicado em: 2003
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33. Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata
Simple sequence repeat anchored polymerase chain reaction amplification (SSR-PCR) is a genetic typing technique based on primers anchored at the 5' or 3' ends of microsatellites, at high primer annealing temperatures. This technique has already been used in studies of genetic variability of several organisms, using different primer designs. In order to condu
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-10
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34. Characterization of Brazilian soybean cultivars using microsatellite markers
Microsatellite markers or SSR (Simple Sequence Repeats) have proved to be an excellent tool for cultivar identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However, these traits have not been sufficient to characterize the large nu
Genetics and Molecular Biology. Publicado em: 2002
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35. Simple Sequence Repeats in Escherichia coli: Abundance, Distribution, Composition, and Polymorphism
Computer-based genome-wide screening of the DNA sequence of Escherichia coli strain K12 revealed tens of thousands of tandem simple sequence repeat (SSR) tracts, with motifs ranging from 1 to 6 nucleotides. SSRs were well distributed throughout the genome. Mononucleotide SSRs were over-represented in noncoding regions and under-represented in open reading fr
Cold Spring Harbor Laboratory Press.
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36. Computational and Experimental Analysis of Microsatellites in Rice (Oryza sativa L.): Frequency, Length Variation, Transposon Associations, and Genetic Marker Potential
A total of 57.8 Mb of publicly available rice (Oryza sativa L.) DNA sequence was searched to determine the frequency and distribution of different simple sequence repeats (SSRs) in the genome. SSR loci were categorized into two groups based on the length of the repeat motif. Class I, or hypervariable markers, consisted of SSRs ≥20 bp, and Class II, or pote
Cold Spring Harbor Laboratory Press.