anÃlise in silico deEST-SSR em phaseolus vulgaris e Glycine max e transferibilidade de marcadores para Vigna unguiculata

AUTOR(ES)

Josà Ribamar Costa Ferreira Neto

DATA DE PUBLICAÇÃO

2008

RESUMO

Expressed Sequence Tags (EST) database is a valuable source of information for molecular markers development. In the present work, simple sequence repeats (SSR) have been detected in Phaseolus vulgaris and Glycine max ESTs, aiming the development of ESTSSR markers for cowpea (Vigna unguiculata) improvement. A total of 10,880 common bean ESTs and 116,965 soy ESTs were analyzed and the estimated averages frequencies were one SSR in every 22.0 kb for common bean EST and one in every 14.7 Kb for soy EST. In common bean ESTs, 331 SSRs were identified and the trimeric motifs were the most abundant (45.6%). In soy ESTs, 4,553 SSRs were detected and the dimeric motifs were the most prevalent (49.7%). In both species, the AG/GA / TC/CT dinucleotide motifs and the AAG/AGA/GAA / TTC/TCT/CTT trinucleotide motifs were the most abundant. A total of 153 common bean EST-SSR primers pairs and 1,928 soy primers pairs were designed. Nine of a 20 soy EST-SSR primers pairs amplified soy DNAs. After transferability study to V. unguiculata, two of these primers pairs amplified but a non-expected size bands with no conserved original motif were observed. On the other hand, 15 of 22 common bean EST-SSR primers pairs amplified in PCR reactions with DNAs from 11 common bean genotypes and 10 of them showed polymorphism (estimated average PIC of 0.50). With cowpea DNAs (17), 11 of these 15 primers pairs amplified several amplicons. Five of them showed polymorphism (estimated average PIC of 0.36). Some PCR products were sequenced and the alignments with the correspondent EST showed in general that the SSR motif extensions modified but the sequences ends were conserved between species. These results confirmed the success of the EST-SSR markers transferability between common beans and cowpea.

ASSUNTO(S)

genetica fabaceae microsatellites data mining microssatÃlite cowpea leguminosa feijÃo-caupi leguminous fabaceae mineraÃÃo de dados

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