Sequencing Chip
Mostrando 1-12 de 35 artigos, teses e dissertações.
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1. Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients
OBJECTIVE: To evaluate the usefulness of DNA methods to provide a means to precisely genotypically match donor blood units for the antigen-negative type of 35 sickle cell disease patients<. METHODS: Red blood cell units were investigated for ABO, D, C, c, E, e, K, Fyª, Fy b, Jkª, Jk b, S, s, Diª and RH variants by performing a molecular array (Human Eryth
Rev. Bras. Hematol. Hemoter.. Publicado em: 2013
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2. Uma abordagem alternativa para seqÃenciamento por hibridizaÃÃo
Uma questÃo central no emergente campo da Biologia Molecular Computacional diz respeito ao problema de seqÃenciamento de DNA. SeqÃenciar uma molÃcula de DNA significa determinar a ordem das suas bases componentes â adenina (A), citosina (C), guanina (G) e timina (T). Em vista do comprimento de tais molÃculas, muitas vezes da ordem de bilhÃes de bases,
Publicado em: 2003
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3. Rapid p53 sequence analysis in primary lung cancer using an oligonucleotide probe array
The p53 gene was sequenced in 100 primary human lung cancers by using direct dideoxynucleotide cycle sequencing and compared with sequence analysis by using the p53 GeneChip assay. Differences in sequence analysis between the two techniques were further evaluated to determine the accuracy and limitations of each method. p53 mutations were either detected by
The National Academy of Sciences.
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4. A gene-specific DNA sequencing chip for exploring molecular evolutionary change
Sequencing by hybridization (SBH) approaches to DNA sequencing face two conflicting constraints. First, in order to ensure that the target DNA binds reliably, the oligonucleotide probes that are attached to the chip array must be >15 bp in length. Secondly, the total number of possible 15 bp oligonucleotides is too large (>415) to fit on a chip with current
Oxford University Press.
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5. Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis
We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was
Oxford University Press.
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6. The Human MitoChip: A High-Throughput Sequencing Microarray for Mitochondrial Mutation Detection
Somatic mitochondrial mutations are common in human cancers, and can be used as a tool for early detection of cancer. We have developed a mitochondrial Custom Reseq™ microarray as an array-based sequencing platform for rapid and high-throughput analysis of mitochondrial DNA. The MitoChip contains oligonucleotide probes synthesized using standard photolitho
Cold Spring Harbor Laboratory Press.
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7. Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3α,4α-diaza-s-indacene (Bodipy)-FL-510, dCTP-PC-Bodipy-650, and dUTP-PC-6-carboxy-X-rhodamine (ROX)
National Academy of Sciences.
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8. Rapid Identification of Probiotic Lactobacillus Biosurfactant Proteins by ProteinChip Tandem Mass Spectrometry Tryptic Peptide Sequencing
A novel ProteinChip-interfaced tandem mass spectrometer was employed to identify collagen binding proteins from biosurfactant produced by Lactobacillus fermentum RC-14. On-chip tryptic digestion of the captured collagen binding proteins resulted in rapid sequence identification of five novel tryptic peptide sequences via collision-induced dissociation tandem
American Society for Microbiology.
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9. Hierarchical hidden Markov model with application to joint analysis of ChIP-chip and ChIP-seq data
Motivation: Chromatin immunoprecipitation (ChIP) experiments followed by array hybridization, or ChIP-chip, is a powerful approach for identifying transcription factor binding sites (TFBS) and has been widely used. Recently, massively parallel sequencing coupled with ChIP experiments (ChIP-seq) has been increasingly used as an alternative to ChIP-chip, offer
Oxford University Press.
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10. Rapid parallel mutation scanning of gene fragments using a microelectronic protein–DNA chip format
We have developed a method for the de novo discovery of genetic variations, including single nucleotide polymorphisms and mutations, on microelectronic chip devices. The method combines the features of electronically controlled DNA hybridisation on open-format microarrays, with mutation detection by a fluorescence-labelled mismatch- binding protein. Electron
Oxford University Press.
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11. Automated parallel DNA sequencing on multiple channel microchips
We report automated DNA sequencing in 16-channel microchips. A microchip prefilled with sieving matrix is aligned on a heating plate affixed to a movable platform. Samples are loaded into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array of electrodes in the focal plane of a four-color scanning detection system. U
The National Academy of Sciences.
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12. Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization.
Oligonucleotide microchips are manufactured by immobilizing presynthesized oligonucleotides within 0.1 x 0.1 x 0.02 mm or 1 x 1 x 0.02 mm polyacrylamide gel pads arranged on the surface of a microscope slide. The gel pads are separated from each other by hydrophobic glass spacers and serve as a kind of 'microtest tube' of 200 pl or 20 nl volume, respectively