Sensitivity And Specificity Of Serological Test
Mostrando 13-24 de 193 artigos, teses e dissertações.
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13. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect i
Mem. Inst. Oswaldo Cruz. Publicado em: 19/02/2016
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14. Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis
Chronic Chagas disease diagnosis relies on laboratory tests due to its clinical characteristics. The aim of this research was to review commercial enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) diagnostic test performance. Performance of commercial ELISA or PCR for the diagnosis of chronic Chagas disease were systematically sea
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-01
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15. Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy
The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detectToxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological screening profiles for toxoplasmosis: unexposed (n = 65), probabl
Mem. Inst. Oswaldo Cruz. Publicado em: 2015-09
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16. Comparison of recombinant A2-ELISA with rKE16 dipstick and direct agglutination tests for diagnosis of visceral leishmaniasis in dogs in Northwestern Iran
INTRODUCTION: Various methods are used for the diagnosis of visceral leishmaniasis (VL), such as microscopic examination, culture and inoculation of laboratory animals; however, serological assays are commonly used for the detection of antibodies in serum samples with a wide range of specificity and sensitivity. METHODS: The purpose of this study was to com
Rev. Soc. Bras. Med. Trop.. Publicado em: 2015-04
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17. Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico
Immunological diagnostic methods for Trypanosoma cruzi depend specifically on the presence of antibodies and parasitological methods lack sensitivity during the chronic and “indeterminate” stages of the disease. This study performed a serological survey of 1,033 subjects from 52 rural communities in 12 of the 18 municipalities in the state of Querétaro,
Mem. Inst. Oswaldo Cruz. Publicado em: 21/10/2014
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18. Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon
Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutinat
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 02/09/2014
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19. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The pre
Braz. J. Microbiol.. Publicado em: 02/05/2014
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20. Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. p
Braz. J. Microbiol.. Publicado em: 15/11/2013
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21. Use of the Kala-Azar Detect® and IT-LEISH® rapid tests for the diagnosis of visceral leishmaniasis in Brazil
The performances of two rapid tests and a standard serological test for the diagnosis of visceral leishmaniasis (VL) were compared using sera from 193 patients with VL and 85 controls. The Kala-Azar Detect®, IT-LEISH® and IFI-LH® assays showed sensitivities of 88.1%, 93.3% and 88.6%, respectively, and specificities of 90.6%, 96.5% and 80%, respectively. T
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-11
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22. PRODUÇÃO DE ANTÍGENOS DO VÍRUS DA ARTRITE ENCEFALITE CAPRINA PARA IMUNODIAGNÓSTICO / PRODUCTION OF VIRUS ANTIGENS OF GOATS FOR ARTHRITIS ENCEPHALITIS IMMUNODIAGNOSTIC
The small ruminantslentiviruses are responsible for a significant economic loss that enabled the development of efficient control and monitoring programs in Europe and other countries.The diagnosis of CAE is mainly based on serological tests.The World Organisation for Animal Health (OIE) recommends the use of agarose gel immunodiffusion (AGID) for its low co
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/07/2011
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23. Malaria diagnosis from pooled blood samples: comparative analysis of real-time PCR, nested PCR and immunoassay as a platform for the molecular and serological diagnosis of malaria on a large-scale
Malaria diagnoses has traditionally been made using thick blood smears, but more sensitive and faster techniques are required to process large numbers of samples in clinical and epidemiological studies and in blood donor screening. Here, we evaluated molecular and serological tools to build a screening platform for pooled samples aimed at reducing both the t
Mem. Inst. Oswaldo Cruz. Publicado em: 2011-09
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24. Standardization of enzyme immunoassay (ELISA) for serology, detection and quantification of porcine circovirus type 2 / Padronização de ensaios imunoenzimáticos (ELISA) para sorologia, detecção e quantificação do circovírus suíno 2
Porcine circovirus 2 (PCV-2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) and is associated with different syndromes that affect the swine. The term PCVAD (Porcine circovirus associated diseases) was introduced in 2006 to gather many diseases. Since serological studies are essential for monitoring the virus, the aim of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/05/2010