Rna Secondary Structure
Mostrando 25-36 de 1566 artigos, teses e dissertações.
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25. Comparative analysis of secondary structure of insect mitochondrial small subunit ribosomal RNA using maximum weighted matching
Comparative analysis is the preferred method of inferring RNA secondary structure, but its use requires considerable expertise and manual effort. As the importance of secondary structure for accurate sequence alignment and phylogenetic analysis becomes increasingly realised, the need for secondary structure models for diverse taxonomic groups becomes more pr
Oxford University Press.
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26. Assay for Secondary Structure in Ribonucleic Acid
A chromatographic procedure is described which can discriminate among single-stranded ribonucleic acid (RNA) molecules in solution on the basis of the extent of their secondary structure. The assay is effected through chromatography at different temperatures with columns of cellulose CF-11. When Sindbis virus RNA is chromatographed in this system, the ratio
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27. Widespread Selection for Local RNA Secondary Structure in Coding Regions of Bacterial Genes
Redundancy of the genetic code dictates that a given protein can be encoded by a large collection of distinct mRNA species, potentially allowing mRNAs to simultaneously optimize desirable RNA structural features in addition to their protein-coding function. To determine whether natural mRNAs exhibit biases related to local RNA secondary structure, a new
Cold Spring Harbor Laboratory Press.
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28. Electron microscopic mapping of secondary structures in bacterial 16S and 23S ribosomal ribonucleic acid and 30S precursor ribosomal ribonucleic acid.
Electron microscopy revealed reproducible secondary structure patterns within partially denatured 16S and 23S ribosomal ribonucleic acid (rRNA) from Escherichia coli. When prepared with 50% formamide-100 mM ammonium acetate, 16S rRNA included two small hairpins that appeared in over 50% of all molecules. Three open loops were observed with frequencies of les
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29. Xenopus laevis 18S ribosomal RNA: experimental determination of secondary structural elements, and locations of methyl groups in the secondary structure model.
18S ribosomal RNA from X. laevis was subjected to partial digestion with ribonucleases A or T1 under a variety of conditions, and base-paired fragments were isolated. Sequence analysis of the fragments enabled five base-paired secondary structural elements of the 18S RNA to be established. Four of these elements (covering bases 221-256, 713-757, 1494-1555 an
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30. Further perspective on the catalytic core and secondary structure of ribonuclease P RNA.
Phylogenetic comparative analyses of RNase P RNA-encoding gene sequences from Chlorobium limicola, Chlorobium tepidum, Bacteroides thetaiotaomicron, and Flavobacterium yabuuchiae refine the secondary structure model of the general (eu)bacterial RNase P RNA and show that a highly conserved feature of that RNA is not essential. Two helices, comprised of 2 base
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31. Transcription termination in vitro at the tryptophan operon attenuator is controlled by secondary structures in the leader transcript.
The role of alternative RNA secondary structures in regulating transcription termination at the attenuator of the tryptophan (trp) operon of Serratia marcescens was examined in vitro by transcribing mutant DNA templates having deletions of different segments of the trp leader region. Deletions that removed sequences corresponding to successive segments of po
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32. A minimal ribosomal RNA: sequence and secondary structure of the 9S kinetoplast ribosomal RNA from Leishmania tarentolae.
The portion of the Leishmania tarentolae kinetoplast maxicircle DNA encoding the 9S RNA gene was sequenced, and the 5' and 3' ends of the transcript were determined. A secondary structure for the 9S RNA was determined based on the Escherichia coli 16S model. The 610-nucleotide 9S RNA exhibits a minimal secondary structure in which all four domains of the E.
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33. Secondary structure of the HIV-2 leader RNA comprising the tRNA-primer binding site.
The initiation of reverse transcription of a retroviral RNA genome occurs by a tRNA primer bound near the 5' end of the genomic RNA at a position called the primer-binding site (PBS). To understand the molecular basis for this RNA-RNA interaction, the secondary structure of the leader RNA of the human immunodeficiency virus type 2 (HIV-2) RNA was analyzed. I
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34. GPRM: a genetic programming approach to finding common RNA secondary structure elements
RNA molecules play an important role in many biological activities. Knowing its secondary structure can help us better understand the molecule's ability to function. The methods for RNA structure determination have traditionally been implemented through biochemical, biophysical and phylogenetic analyses. As the advance of computer technology, an increasing n
Oxford University Press.
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35. Secondary structure of eukaryotic cytoplasmic 5S ribosomal RNA.
A five-helix secondary structural model is proposed for eukaryotic cytoplasmic 5S rRNA. All available sequence data are consistent with this model including those from Chlorella 5S rRNA whose sequence is revised by data included here. Various architectural features of eukaryotic 5S rRNA are summarized in terms of this secondary structural model. It is observ
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36. RNA folding causes secondary structure rearrangement
The secondary structure of the P5abc subdomain (a 56-nt RNA) of the Tetrahymena thermophila group I intron ribozyme has been determined by NMR. Its base pairing in aqueous solution in the absence of magnesium ions is significantly different from the RNA in a crystal but is consistent with thermodynamic predictions. On addition of magnesium ions, the RNA fold
The National Academy of Sciences.