Rna Interference
Mostrando 25-36 de 1214 artigos, teses e dissertações.
-
25. Arginine induces GH gene expression by activating NOS/NO signaling in rat isolated hemi-pituitaries
The amino acid arginine (Arg) is a recognized secretagogue of growth hormone (GH), and has been shown to induce GH gene expression. Arg is the natural precursor of nitric oxide (NO), which is known to mediate many of the effects of Arg, such as GH secretion. Arg was also shown to increase calcium influx in pituitary cells, which might contribute to its effec
Braz J Med Biol Res. Publicado em: 2012-11
-
26. Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediati
Genetics and Molecular Biology. Publicado em: 05/07/2012
-
27. Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantatio
Genetics and Molecular Biology. Publicado em: 2012
-
28. Análise de genes envolvidos no modelo de epilepsia de lobo temporal induzido pela pilocarpina / Analysis of genes involved in the temporal lobe epilepsy induced by pilocarpine
The epilepsies affect approximately 1% of the population worldwide. Temporal lobe epilepsy (TLE) is the most frequent and commonly resistant to clinical treatment. Animal models have been widely used in order to study the mechanisms underlying TLE, especially those induced by chemical agents or electrical stimulation. It is believed that these models present
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/11/2011
-
29. A anêmona-do-mar Diadumene Stephenson, 1920: um cnidário com potencial invasor na Baía de Paranaguá, PR, Brasil / The sea anemone Diadumene Stephenson, 1920: a cnidarian with an invasion potential in Paranaguá Bay, PR, Brazil
A great number of sea anemones of the genus Diadumene Stephenson, 1920 were found fixated on natural and artificial substrates at the Paranaguá Bay. In 1989, in a study conducted at the same location, the number of anemones of this genus, not identified to a specific level, was much lower. The great abundance of anemones that has currently been observed may
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/09/2011
-
30. Caracterização de determinantes genéticos envolvidos na qualidade industrial e nutricional do fruto de tomate / Characterization of genetic determinants involved in industrial and nutritional quality of tomato fruit
This thesis addresses tomato fruit metabolism analysis by using three complementary approaches: i) the identification of candidate genes co-located with QTL previously described from metabolic profiles of S. lycopersicum (cv. M82) and S. pennellii introgressed lines, associated with industrial and nutritional quality traits; ii) the structural analyses of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011
-
31. Análise funcional de genes expressos em glândula esofágica dorsal de meloidogyne incógnita envolvidos no fitoparasitismo
Os fitonematóides do gênero Meloidogyne estão entre os mais prejudiciais para a economia agrícola mundial e são intensamente estudados devido aos danos causados a uma grande variedade de culturas agronomicamente importantes. Técnicas convencionais de controle como rotação de culturas e uso de nematicidas têm se mostrado ineficazes e danosas, fazendo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/07/2011
-
32. Focal Adhesion Kinase is critical for the expression of pro-atherogenic molecules in vascular cells subjected to mechanical stress / Quinase de adesão focal é crítica para a expressão de moléculas pró-aterogênicas em células vasculares submetidas a estresse mecânico
O aumento do estresse circunferencial ou mecânico é um dos principais estímulos responsáveis pela aterogênese induzida por hipertensão arterial, além de ser um determinante para a localização das placas ateroscleróticas na árvore arterial. Neste contexto, moléculas mecano-sensíveis ou responsivas ao estresse mecânico podem exercer um papel fund
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/07/2011
-
33. Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma
In order to investigate signal transduction and activation of transcription 3 (STAT3) signaling on angiogenesis in colorectal carcinoma (CRC) after inhibiting STAT3 expression, we constructed the HT-29-shSTAT3 cell line by lentivirus-mediated RNAi. Cell growth was assessed with MTT and the cell cycle distribution by flow cytometry. CRC nude mouse models were
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-12
-
34. Progress with schistosome transgenesis
Genome sequences for Schistosoma japonicum and Schistosoma mansoni are now available. The schistosome genome encodes ~13,000 protein encoding genes for which the function of only a minority is understood. There is a valuable role for transgenesis in functional genomic investigations of these new schistosome gene sequences. In gain-of-function approaches, tra
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
-
35. Inhibition of cytohesin-1 by siRNA leads to reduced IGFR signaling in prostate cancer
To explore how cytohesin-1 (CYTH-1) small interfering RNA (siRNA) influences the insulin-like growth factor receptor (IGFR)-associated signal transduction in prostate cancer, we transfected human prostate cancer PC-3 cell lines with liposome-encapsulatedCYTH-1 siRNA in serum-free medium and exposed the cells to 100 nM IGF-1. The mRNA and protein levels of th
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-07
-
36. CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of
Braz J Med Biol Res. Publicado em: 2011-03