Rna Interference
Mostrando 13-24 de 1214 artigos, teses e dissertações.
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13. Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and inva
Braz J Med Biol Res. Publicado em: 23/05/2014
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14. CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo.
Braz J Med Biol Res. Publicado em: 21/03/2014
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15. The new world of RNAs
One of the major developments that resulted from the human genome sequencing projects was a better understanding of the role of non-coding RNAs (ncRNAs). NcRNAs are divided into several different categories according to size and function; however, one shared feature is that they are not translated into proteins. In this review, we will discuss relevant aspec
Genet. Mol. Biol.. Publicado em: 2014
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16. Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)
In this work, a bivalent RNA interference (RNAi) plant-transformation vector was constructed to silence both the flavanone 3-hydroxylase (F3H) gene and the flavone synthase II (GmFNSII) gene in soybean (Glycine max). Two further unit RNAi vectors were constructed for each of these two genes. RNAi-mediated suppression of these genes effectively regulated flav
Braz. arch. biol. technol.. Publicado em: 26/11/2013
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17. In vitro and in vivo inhibition of rabies virus replication by RNA interference
Rabies is a zoonotic disease that affects all mammals and leads to more than 55,000 human deaths every year, caused by rabies virus (RABV) (Mononegavirales: Rhabdoviridae: Lyssavirus). Currently, human rabies treatment is based on the Milwaukee Protocol which consists on the induction of coma and massive antiviral therapy. The aim of this study was to assess
Braz. J. Microbiol.. Publicado em: 15/11/2013
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18. Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw
Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key en
Genet. Mol. Biol.. Publicado em: 25/10/2013
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19. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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20. Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference
Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or
Mem. Inst. Oswaldo Cruz. Publicado em: 2013
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21. Inibição simultânea dos genes antiapoptóticos Bcl-2 e Bcl-XL em células de leucemia linfoide aguda e células de linfoma do manto mediante RNA de interferência / Simultaneous inhibition of antiapoptóticosBcl-2 and Bcl-XL genes acute lymphocytic leukemia and mantle cell lymphoma by RNA interference
As estatísticas relacionadas aos cânceres hematológicos indicam que a incidência e mortalidade dessas doenças têm aumentado ao longo dos anos. Embora a maioria dos casos de linfomas e leucemias não possua etiologia definida, sugere-se que fatores genéticos possam estar envolvidos. Nesse contexto, destaca-se a família de proteínas Bcl-2, divididas e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/11/2012
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22. Validação da via de biossíntese de selenocisteína e selenoproteínas em Trypanosoma por RNA de interferência
Selênio (Se) é um elemento essencial encontrado em selenoproteínas na forma do 21 aminoácido selenocisteína (Sec U). A incorporação co-traducional de Sec depende de uma complexa via de síntese, de um códon de terminação UGA em fase de leitura e uma estrutura terciária do RNA mensageiro conhecida como elemento SECIS. A maioria das selenoproteínas
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/04/2012
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23. Silenciamento do gene da enzima PIPKII alfa em células de linhagem eritroleucêmica humana (K562) / Silencing of the PIPKII alfa in human erythroleukemia cell line
As fosfatidilinositol-fosfato quinases (PIPKs) pertencem a uma família de enzimas lipídio-quinases que geram vários mensageiros lipídicos, incluindo um importante segundo mensageiro denominado fosfatidilinositol-4,5-bifosfato, que participa da regulação de diversas atividades celulares, como a modulação do citoesqueleto de actina, o transporte de ves
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/01/2012
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24. Analysis of energetically biased transcripts of viruses and transposable elements
RNA interference (RNAi) is a natural endogenous process by which double-stranded RNA molecules trigger potent and specific gene silencing in eukaryotic cells and is characterized by target RNA cleavage. In mammals, small interfering RNAs (siRNAs) are the trigger molecules of choice and constitute a new class of RNA-based antiviral agents. In an efficient RNA
Genet. Mol. Biol.. Publicado em: 13/11/2012