Red Blood Cell Folate
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. Concentrations of blood folate in Brazilian studies prior to and after fortification of wheat and cornmeal (maize flour) with folic acid: a review
BACKGROUND: In July 2004, the Brazilian Ministry of Health through the National Health Surveillance Agency made the fortification of wheat flour and cornmeal (maize flour) with iron and folic acid mandatory, with the intention of reducing the rate of diseases such as neural tube defects. OBJECTIVE: The aim of the study was to investigate the impact of
Rev. Bras. Hematol. Hemoter.. Publicado em: 2014-07
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2. Consumo alimentar e concentraÃÃes intraeritrocitÃrias de folato em mulheres em idade reprodutiva do Recife/PE, apÃs a regulamentaÃÃo da fortificaÃÃo das farinhas de trigo e milho com ferro e Ãcido fÃlico
O folato à uma vitamina que desempenha importantes funÃÃes no metabolismo humano. A compreensÃo do seu papel metabÃlico vem ganhando destaque na SaÃde PÃblica. Mulheres em idade reprodutiva constituem um grupo vulnerÃvel a deficiÃncia de folato. A fortificaÃÃo de alimentos com Ãcido fÃlico tem sido apontada como uma alternativa para promover aum
Publicado em: 2009
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3. Measurement of red cell folate with 75Se-selenofolate radioassay.
75Se-selenofolate radioassay was adapted for the purpose of red cell folate measurement. The results of assay of 124 whole blood samples by Lactobacillus casei microbiological assay and by radioassay using the adapted selenofolate method were concordant (r = 0.8). Red cell folate levels by the radioassay were below 170 microgram/1 in people who had haematolo
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4. Importance of low serum vitamin B12 and red cell folate concentrations in elderly hospital inpatients.
To determine the functional importance of the low B12 and red cell folate concentrations repeatedly observed in the elderly 200 consecutive patients admitted to a geriatric unit were studied. Forty six of the patients had low serum concentrations of B12 (15), red cell folate (26), or both (five). Serum B12 and red cell folate concentrations correlated with m
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5. A common mutation in the 5,10-methylenetetrahydrofolate reductase gene affects genomic DNA methylation through an interaction with folate status
DNA methylation, an essential epigenetic feature of DNA that modulates gene expression and genomic integrity, is catalyzed by methyltransferases that use the universal methyl donor S-adenosyl-l-methionine. Methylenetetrahydrofolate reductase (MTHFR) catalyzes the synthesis of 5-methyltetrahydrofolate (5-methylTHF), the methyl donor for synthesis of methionin
National Academy of Sciences.
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6. Automation of the assay of folate in serum and whole blood
An automated system is described for the microbiological assay of serum and red cell folate concentrations using a chloramphenicol-resistant strain of L. casei. Both serum and whole-blood haemolysates are assayed directly without previous deproteinization. Results by this method are comparable to those obtained by standard manual folate assays. Reproducibili
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7. Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency
A simplified microbiological assay for determining the folate content of red cells is described. As in previously reported methods Lactobacillus casei is used as test organism but two modifications are introduced. First, haemolysis is carried out in water containing 1 g.% of ascorbic acid; secondly, haemolysates are not incubated before the assay. Using this
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8. Deriving a useful packed cell volume estimate from haemoglobin analysis.
A method is described whereby the packed cell volume (PCV) of a blood sample can be estimated from the haemoglobin concentration. The method defines the relation existing between haemoglobin concentration and the PCV of blood samples and uses the mathematical formula thus obtained to estimate an unknown PCV value for a sample by extrapolation from the known
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9. A common mutation in the methylenetetrahydrofolate reductase gene is associated with an accumulation of formylated tetrahydrofolates in red blood cells
A common mutation (C677T) in the gene encoding for methylenetetrahydrofolate reductase (MTHFR) (5-methyltetrahydrofolate:(acceptor) oxidoreductase, EC 1.7.99.5), a key regulatory enzyme in one-carbon metabolism, results in a thermolabile variant of the MTHFR enzyme with reduced activity in vitro. In the present study we used a chromatographic method for fola
The National Academy of Sciences.
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10. Use of plasma ferritin concentration to diagnose iron deficiency in elderly patients.
AIMS--To determine a concentration of ferritin below which the possibility of iron deficiency should be considered in elderly patients. METHODS--Consecutive new referrals to a geriatric unit (n = 472) were studied prospectively. Full blood count, ferritin, serum vitamin B12 and red cell folate were measured for all patients. A blood film was assessed indepen
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11. Karyotype 69,XXX/47,XX,+15 in a 2 1/2 year old child.
We present the clinical findings in a 2 1/2 year old girl with an unusual mosaic karyotype. Amniocentesis was performed at 35 weeks because of intrauterine growth retardation. The in situ cultures showed 47,XX,+15 in seven colonies, 69,XXX in four colonies, and in two colonies 46,XX was detected. Subcultures showed 69,XXX/47,XX,+15 with no normal cells. A sm