Protoplasts
Mostrando 1-12 de 1333 artigos, teses e dissertações.
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1. Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.
Resumo Protoplastos in vivo do mesofilo foliar da cultivar de macieira “Anna” foram isolados de folhas com 15 dias de idade através de plasmólise em meios contendo manitol a 90 g L-1, por meia hora e em seguida a 130 g L-1, pelo mesmo tempo. Anteriormente ao isolamento dos protoplastos foi realizado um pré tratamento com mistura enzimática contendo
Rev. Bras. Frutic.. Publicado em: 05/08/2019
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2. A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis
Abstract Chloroplast development and chlorophyll (Chl) biosynthesis in plants are regulated by many genes, but the underlying molecular mechanisms remain largely elusive. We isolated a rice mutant named yss2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth. The mutant developed normal green leaves from
Genet. Mol. Biol.. Publicado em: 31/08/2017
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3. Transformação de plantas.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain
Planaltina. Publicado em: 2011
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4. A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cu
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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5. Obtenção de híbridos somáticos de limão 'Cravo' e tangerina 'Cleópatra'.
Este trabalho teve como objetivo a obtenção de híbridos somáticos entre o limão 'Cravo' (Citrus limonia Osbeck) e a tangerina 'Cleópatra' (Citrus reshni Hort.), para serem usados como porta-enxertos de citros. O limão 'Cravo' é atualmente o principal porta-enxerto comercial utilizado no Brasil, em virtude de suas boas qualidades agronômicas. Entreta
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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6. Hibridação somática visando à produção de genitores tetraplóides para o melhoramento de cultivares copa de citros / Somatic hybridization in order to obtain tetraploid parents for citrus scion improvement
A hibridação somática via fusão de protoplastos é um método de combinação genética que agrega integralmente os dois genomas genitores, assim como suas respectivas organelas citoplasmáticas, sendo uma ferramenta alternativa aos métodos convencionais de melhoramento. O objetivo deste trabalho foi a obtenção de híbridos somáticos interespecífico
Publicado em: 2011
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7. Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.)
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively
Brazilian Journal of Microbiology. Publicado em: 2010-03
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8. Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
O objetivo deste trabalho foi padronizar as condições de obtenção e regeneração de protoplastos de Epulorhiza repens e Ceratorhiza sp. Para o fungo E. repens, a maior produção de protoplastos, 8.0 x 10(6) protoplastos/mL, foi obtida em KCl 0.6 M, na presença de 15 mg/mL de "Lysing Enzymes" e micélio fúngico com 2 dias de idade. A maior freqüênci
Brazilian Archives of Biology and Technology. Publicado em: 2010-02
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9. Glass bead transformation method for gram-positive bacteria
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
Brazilian Journal of Microbiology. Publicado em: 2009-12
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10. TransformaÃÃo de Stenocarpella maydis com os genes marcadores GFP e DsRed e patogenicidade dos transformados em sementes de milho / Transformation of Stenocarpella maydis with genes markers GFP and DsRed and pathogenicity of transformed into maize seeds
One of the fungi which can associate to maize seeds is Stenocarpella maydis, causal agent of stalk and ear rot, which is responsible by considerable losses in producing regions of this crop in Brazil. From literature, there is little information on the mechanisms of infection and on the transmission dynamics of this fungus in relation to maize seeds. For thi
Publicado em: 2009
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11. Identificação de genes expressos durante a fase pré-simbiótica da associação ectomicorrízica entre Hydnangium sp. e Eucalyptus grandis e transformação de fungos ectomicorrízicos / Identification of genes expressed during the pre-symbiotic interaction in the Hydnangium sp.-Eucalyptus grandis ectomycorrhizal association and transformation of ectomycorrhizal fungi
Gene expression in the ectomycorrhizal fungus Hydnangium sp. during the pre-symbiotic interaction was evaluated using an in vitro mycorrhization technique aiming at constructing a suppressive subtractive cDNA library. The fungus was cultivated in the presence of Eucalyptus grandis roots, with no direct physical contact between both organisms. Genes that code
Publicado em: 2008
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12. Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana / Study of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamiana
Begomoviruses belong to the family Geminiviridae, which includes viruses with a circular, single-stranded DNA genome encapsidated in twinned icosahedral particles. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and cause serious diseases in several economically important crops, mostly in tropical and subtropical regions. In Brazil, a viral com
Publicado em: 2008