Protein Refolding
Mostrando 1-12 de 208 artigos, teses e dissertações.
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1. Investigações estruturais dos domínios funcionais das miosinas classes VIII e XI presentes em plantas / Structural investigations of the functional domains of plant myosins (classes VIII and XI)
As miosinas formam uma superfamília de proteínas de alto peso molecular com atividade mecanoquímica capaz de hidrolisar a molécula de ATP e de interagir com os filamentos de actina. A estrutura das miosinas pode ser divida de modo geral em cabeça motora, pescoço e cauda. São conhecidas 35 classes de miosinas em eucariotos sendo a classe II de miosinas
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
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2. Expressão heteróloga de neurotoxinas do veneno da aranha Phoneutria nigriventer
The venom of the spider Phoneutria nigriventer contains several peptides that act on ion channels and receptors of the nervous system of insects and mammals. The toxins Tx3-6 and Tx3-4 are voltage-gated calcium channel blockers and have shown a pharmacological potential in the treatment of pain and in neuroprotection. Only small amounts of these toxins can b
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/06/2011
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3. Expressão e caracterização de proteínas envolvidas na via da quinase mTOR e na divisão celular bacteriana / Expression and characterization of proteins involved in the mTOR kinase pathway and bacterial cell division
A mTOR é uma via de sinalização muito conservada que controla o crescimento celular em resposta à presença de nutrientes e fatores de crescimento. A desregulação dessa via em humanos está relacionada a doenças como câncer e diabetes. A quinase TOR é ativada na presença de aminoácidos e recentemente descobriu-se que as pequenas GTPases da famíli
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/02/2011
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4. Construção e análise das propriedades profiláticas e terapêuticas de uma vacina contra tumores associados ao HPV-16. / Construction and analysis of the prophylactic and therapeutic proprieties of a vaccine against HPV-16 associated tumors.
The development of vaccines against human papillomavirus (HPV) represents an important alternative to control the sexually transmitted infection and cervical cancer. In this paper we explored a novel vaccine strategy against tumors induced by HPV-16 using the E7 protein obtained after genetic fusion to the glycoprotein D of herpes virus type 1, a protein wit
Publicado em: 2010
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5. Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em Escherichia coli. / Expression of recombinant human colony stimulating factor (rhG-CSF) in Escherichia coli.
The recombinant human granulocyte colony stimulating factor (rhG-CSF) is a non-glycosylated protein with 175 amino acids. This factor plays an important role in hematopoietic cell proliferation and has been widely used for treating neutropenia. The purpose of this work is to construct two expression systems in E. coli; a system for obtaining rhG-CSF in the c
Publicado em: 2010
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6. REFOLDING IN HIGH HIDROSTATIC PRESSURE OF RECOMBINANT PROTEINS FROM INCLUSION BODIES IN ESCHERICHIA Coli / Renaturação em altas pressões hidrostáticas de proteínas recombinantes agregadas em corpos de inclusão produzidos em Escherichia coli
The expression of proteins as inclusion bodies in bacteria is a widely used alternative for production of recombinante protein. However, the aggregation is a problem often encountered during refolding of these proteins. High hydrostatic pressure are able to solubilize the inclusion bodies in the presence of low concentrations of denaturant reagents, encourag
Publicado em: 2009
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7. Structural studies of the proteins Q4DV70 from Trypanosoma cruzi and mesothelin from Homo sapiens / Estudos estruturais das proteinas Q4DV70 de Trypanosoma cruzi e mesotelina de Homo sapiens
In this work we carried out studies with two proteins, Q4DV70 from Trypanosoma cruzi and mesothelin from Homo sapiens. The aim was to help the understanding of the function of these proteins which possibly are important to Chagas disease and cancer, respectively. Q4DV70 protein was annotated in T. cruzi genome as a conserved hypothetical protein. In our stud
Publicado em: 2009
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8. Caracterization of a thaumatin-like gene expressed by Moniliophthora Perniciosa fungus, the causative agent of cacao Whiches Broom disease / Caracterização de um gene similar a taumatina expresso pelo fungo causador da Vassoura de bruxa, Moniliophthora perniciosa
Moniliophthora perniciosa is a phytopatogen that has economical importance in Brazil, particularly in the south of Bahia, because this fungus causes the disease in cacao known as Witches Broom disease. This fungus is a hemibiotrofic pathogen that has two distinct stages: a biotrophic (or parasitic) and a necrotrophic (or saprotrophic). The database generated
Publicado em: 2008
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9. Isolamento e caracterização da lectina camptosemina extraída das sementes de Camptosema ellipticum
Lectins are (glico) proteins of non immune origin able to cause cellular agglutination or precipitation of glicoconjugates. Legume lectin refers to plant lectins that are found exclusively in species of the Leguminosae family. A notable characteristic of legume lectins is that all their proteins share tertiary structure consisting of a jelly-roll motif, whic
Publicado em: 2007
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10. Clonagem e expressão do cDNA codificante para a toxina do veneno de Lasiodora sp, LTx2, em vetor de expressão pET11a.
The venom from snakes, scorpions, spiders and other venomous animals is a very rich source of potent toxins. An interesting feature of some toxins is their remarkable species specificity. In the present work we cloned a sequence which codifies for the toxin LTx2 from the venom of the spider Lasiodora sp, in the expression vector pET11a. This toxin was previo
Publicado em: 2006
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11. Expressão heteróloga e caracterização bioquímica da proteína recombinante GmSBP2/S64 da soja (Glycine max) / Heterologous expression and biochemical characterization of GmSBP2/S64 recombinant protein from soybean (Glycine max)
The sucrose binding protein (SBP) belongs to the cupin family of proteins and is structurally related to vicilin-like storage proteins. In this investigation, SBP was expressed in E. coli and large amounts of the protein accumulated in the insoluble fraction as aggregated, denatured protein. The refolding of the purified protein proceeded with a progressive
Publicado em: 2006
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12. Estudos biológicos e estruturais da BthaTL, uma serinopeptidase recombinante da peçonha de Bothrops alternatus (Viperidae, Crotalinae)
Snake venom serine proteinases usually act on the mammal hemostatic system, sometimes resembling the action of trombin, and these enzymes became objects of therapeutical interest against trombosis. In this work we describe the cloning, expression in bacterial system and partial purification of BthaTL, a recombinant serine peptidase with 233 amino acid residu
Publicado em: 2005