Construção e análise das propriedades profiláticas e terapêuticas de uma vacina contra tumores associados ao HPV-16. / Construction and analysis of the prophylactic and therapeutic proprieties of a vaccine against HPV-16 associated tumors.

AUTOR(ES)

Bruna Felicio Milazzotto Maldonado Porchia

DATA DE PUBLICAÇÃO

2010

RESUMO

The development of vaccines against human papillomavirus (HPV) represents an important alternative to control the sexually transmitted infection and cervical cancer. In this paper we explored a novel vaccine strategy against tumors induced by HPV-16 using the E7 protein obtained after genetic fusion to the glycoprotein D of herpes virus type 1, a protein with adjuvant properties for T lymphocytes. The recombinant protein gDE7 was expressed in bacteria and in insect cells and purified by affinity chromatography. The protein generated in bacteria was administered in soluble and insoluble forms as a vaccine in mice. The insoluble gDE7 gave 80% prophylactic protection for tumor growth, the gDE7 that kept the soluble form after refolding protected 100% of the animals under the same conditions. The therapeutic protection reached 30% of the animals. We also verified the neutralizing potential of sera generated against the HSV-1. The conditions for obtaining the proteins expressed in insect cells were also established.

ASSUNTO(S)

glicoproteínas d hpv-16 vaccines hpv-16 glycoproteins d neoplasms purificação de proteínas neoplasias vacinas protein purification

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