Protein Crystallography
Mostrando 25-36 de 198 artigos, teses e dissertações.
-
25. Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals
Reconstitution of membrane proteins into lipid bilayers is a powerful tool to analyze functional as well as structural areas of membrane protein research. First, the proper incorporation of a purified membrane protein into closed lipid vesicles, to produce proteoliposomes, allows the investigation of transport and/or catalytic properties of any membrane prot
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-07
-
26. Determination of the crystallographic structure of the human glucose-6-phosphate isomarase by x-ray diffraction / Determinação da estrutura cristalográfica por difração de raios-x da enzima glicose 6-fosfato isomerase humana
This work is presented as part of the Master degree requirements of the Applied Physics program, Biomolecular Physics area. The purpose of this work is the structural study by of the human glucose-6-phosphate isomerase (PGI-hum). This work has involved mainly three areas: Molecular Biology, Biochemistry and Crystallography. The Molecular Biology work was int
Publicado em: 2001
-
27. Structural, functional and immunological studies on a polymeric bacterial protein
The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
-
28. Structural studies of the scrapie prion protein by electron crystallography
Because the insolubility of the scrapie prion protein (PrPSc) has frustrated structural studies by x-ray crystallography or NMR spectroscopy, we used electron crystallography to characterize the structure of two infectious variants of the prion protein. Isomorphous two-dimensional crystals of the N-terminally truncated PrPSc (PrP 27-30) and a miniprion (PrPS
The National Academy of Sciences.
-
29. Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin
Although hydrogens comprise half of the atoms in a protein molecule and are of great importance chemically and structurally, direct visualization of them by using crystallography is difficult. Neutron crystallography is capable of directly revealing the position of hydrogens, but its use on unlabeled samples faces certain technical difficulties: the large in
The National Academy of Sciences.
-
30. Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods
We describe the use of racemic crystallography to determine the X-ray structure of the natural product plectasin, a potent antimicrobial protein recently isolated from fungus. The protein enantiomers l-plectasin and d-plectasin were prepared by total chemical synthesis; interestingly, l-plectasin showed the expected antimicrobial activity, while d-plectasin
Wiley Subscription Services.
-
31. Purification, crystallization and preliminary X-ray diffraction of wild-type and mutant recombinant human transforming growth factor β-induced protein (TGFBIp)
Wild-type and mutant recombinant human transforming growth factor β-induced protein (TGFBIp) were cloned, purified and crystallized. Preliminary X-ray crystallography data were obtained from wild-type TGFBIp.
International Union of Crystallography.
-
32. Visualization of beta-sheets and side-chain clusters in two-dimensional periodic arrays of streptavidin on phospholipid monolayers by electron crystallography.
The biotin-binding protein streptavidin was crystallized as two-dimensional periodic arrays on biotinylated phospholipid monolayers. Electron diffraction patterns and images of the arrays embedded in vitreous ice were recorded to near-atomic resolution. Amplitudes and phases of structure factors were computed and combined to produce a 3 A projection density
-
33. Crystallization and preliminary X-ray diffraction studies of the calcium-binding protein CalD from Streptomyces coelicolor
The calcium-binding protein encoded by the CalD gene in S. coelicolor A3(2) has been crystallized as a prelude towards the determination of its three-dimensional structure by X-ray crystallography.
International Union of Crystallography.
-
34. There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization
Crystals of an E. coli AcrB contaminant were grown from 95% pure CorA preparations. This very frequently occurring problem in membrane-protein crystallography laboratories is reported, as well as suggestions to avoid it.
International Union of Crystallography.
-
35. Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45γ
The human Gadd45γ protein has been crystallized as a prelude towards determination of its three-dimensional structure by X-ray crystallography.
International Union of Crystallography.
-
36. The three-dimensional structure of retinol-binding protein.
The complex of retinol with its carrier protein, retinol-binding protein (RBP) has been crystallized and its three-dimensional structure determined using X-ray crystallography. Its most striking feature is an eight-stranded up-and-down beta barrel core that completely encapsulates the retinol molecule. The retinol molecule lies along the axis of the barrel w