Protein Crystallography
Mostrando 13-24 de 198 artigos, teses e dissertações.
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13. Estudos estruturais do domínio GTPase isolado da septina humana SEPT4 e estrutura cristalográfica da Glutationa -S- Transferase de Xylella fastidiosa / Structural Studies of the GTPase domain from human SEPT4 and Crystallography Structure of Glutathione S-transferase from Xylella fastidiosa
The septins are a conserved family of guanine nucleotides-binding and hetero-filament forming. proteins. In mammals they are involved in a variety of cellular processes, such as cytokinesis, exocytosis, and vesicle trafficking. SEPT4 has been reported to accumulate in tau-based filamentous deposits and cytoplasmic inclusions in Alzheimers and Parkinsons dise
Publicado em: 2008
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14. Estrutura cristalográfica da enzima superóxido dismutase de Trypanosoma brucei e análise da especificidade do metal incorporado por acoplamento estatístico / Crystal structure of the superoxide dismutase enzyme from Trypanosoma brucei and incorporated metal specificity analysis by statistical coupling.
A doença do sono é causada pelo parasita Tripanosoma brucei. Considerada uma doença negligenciada, mata milhares de pessoas todos os anos na África subsaariana. O T.brucei não apresenta resposta imune pronunciada, o que dificulta o desenvolvimento de vacinas, e os medicamentos disponíveis são escassos. Os tripanossomatídeos são comprovadamente sens�
Publicado em: 2008
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15. Expressão, purificação e caracterização estrutural dos fatores de transcrição bZIP SCF12 e SCF5 de cana-de-açucar / Expression, purification and structural characterization of the sugarcane bZIP transcription factors SCF12 and SCF5
The bZIP transcription factors are present in eukaryotic organisms and are involved in the regulation of gene expression and many intracellular processes. These factors bind specific DNA sequences and are able to recognize regulatory sequences of a gene promoter. The bZIPs are characterized by a conserved region rich in basic amino acid residues as well as b
Publicado em: 2008
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16. Clonagem, expressão e purificação da proteína ligadora de alcano sulfonatos do sistema de transporte ABC de Xanthomonas axonopodis pv.citri. / Cloning, expression and purification of the Xanthomonas axonopodis pv citri ABC transport alkanosulphonate-binding protein.
O genoma de Xanthomonas citri (Xac) possui mais de 20 tipos de transportadores do tipo ABC incluindo o operon ssuABC associado ao transporte de alcano sulfonatos. Deste operon, escolhemos a proteína periplasmática ligadora de alcano sulfonatos SsuA2, para caracterização e análises espectroscópicas e estruturais. A rSsuA2 foi expressa no citoplasma de c
Publicado em: 2008
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17. Estudos estruturais e funcionais das oxidoredutases de pontes dissulfeto da familia DsbA de Xylella fastidiosa / Structural and functional studies of the disulfide oxidorecdutases DsbA from Xylella fastidiosa
As oxidoredutases de pontes dissulfeto da família DsbA são responsáveis pela catálise da formação de pontes dissulfeto em proteínas secretadas para o periplasma, participando do processo de enovelamento de fatores de virulência de diversos organismos. É a proteína com maior potencial de oxidação atualmente caracterizada e tal propriedade é assoc
Publicado em: 2008
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18. Glutamate receptors as seen by light: spectroscopic studies of structure-function relationships
Ionotropic glutamate receptors are major excitatory receptors in the central nervous system and also have a far reaching influence in other areas of the body. Their modular nature has allowed for the isolation of the ligand-binding domain and for subsequent structural studies using a variety of spectroscopic techniques. This review will discuss the role of s
Brazilian Journal of Medical and Biological Research. Publicado em: 2007-11
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19. Investigation of a novel Bacillus thuringiensis toxin
The aim of the present work was to investigate the toxicity, biochemical characteristics, gene and protein contents, and the mechanism of action of toxins from the Brazilian Bacillus thuringiensis (Bt) strain S725. This strain has been reported as toxic against the cotton boll weevil (Anthonomus grandis), a severe pest of cotton crops in America, which is co
Publicado em: 2006
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20. Observance of polymorphic behaviour during dissolution of insulin and lysozyme
Although protein crystallization is a unit operation with potentially high separation factors, it has not been widely used in industry. Protein crystallization studies and practices have hitherto been largely limited to crystallography protocols. Knowledge of the behaviour of protein in solution would help to overcome empiric limitations in protein crystalli
Brazilian Journal of Chemical Engineering. Publicado em: 2005-09
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21. Structural studies of the thyroid receptor (TR) and homology modeling of the thyroxine binding globulin (TBG) / Estudos estruturais do receptor do hormônio tireoidiano (hTR) e modelagem por homologia da globulina de ligação à tiroxina (TBG)
The thyroid hormones are involved in various regulatory effects, on diverse organs. Their fluctuations on the body are related to clinical scenarios of great relevance. This work deals with the study of two proteins which are directly related to the regulatory system associated to these hormones. The first one is the thyroxine-binding globulin (TBG), respons
Publicado em: 2005
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22. Structural studies of the sugarcane enzyme phosphoribosylpyrophosphate synthase / Estudos estruturais da enzima fosforribosilpirofosfato sintase de cana-de-açúcar
A fosforribosilpirofosfato sintase de cana-de-açúcar [sPRS] (EC: 2.7.6.1) é uma enzima de central importância em muitas vias metabólicas envolvida na produção do 5-fosforribosil-1-pirofostato [PRPP] a partir da ribose-5-fosfato [R5F]. O gene da sPRS, seqüenciado como parte do projeto SUCEST, codifica para uma proteína com 328 aminoácidos e massa mo
Publicado em: 2004
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23. Estudos estruturais sobre a Beta-manosidase de Trichoderma reesei e a Triose fosfato isomerase de musculo de coelho
This PhD thesis summarizes the results obtained during the PhD program of the author. The main project consisted in structural studies of b-mannosidase from Trichoderma reesei and rabbit muscle Triose phosphate isomerase (TIM). Protein Crystallography and Small-Angle X-ray Scattering have been the mostly used techniques. The main results are the low resoluti
Publicado em: 2003
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24. Crystallographic structure of the trypsin inhibitor purified from Enterolobium contortisiliquum seeds and the molecular modeling of its complexes with trypsin, trombin and Xa factor. / Estrutura cristalográfica do inibidor de tripsina purificada de sementes de Enterolobium contortisiloquum e modelagem molecular de seus complexos com tripsina, trombina e fator Xa.
The principal objective of this work was the 3D structural determination of a Trypsin inhibitor from Enterolobium contortisiliquum (EcTI) seeds as well as the modeling of the complexes between EcTI and trypsin, chymotrypsin, thrombin and factor Xa and their subsequent analysis. The inhibitor has a molecular weight of 19.5 kDa and inhibits trypsin, chymotryps
Publicado em: 2003