Polysaccharide Purification
Mostrando 13-24 de 86 artigos, teses e dissertações.
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13. CAPSULAR POLYSACCHARIDE OF AZOTOBACTER AGILIS1
Cohen, Gary H. (University of Vermont, Burlington), and Donald B. Johnstone. Capsular polysaccharide of Azotobacter agilis. J. Bacteriol. 88:1695–1699. 1964.—Capsular polysaccharide from Azotobacter agilis strain 132 was recovered from washed cells by alkaline digestion. The polysaccharide was purified by centrifugation, repeated alcohol precipitation, S
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14. Host-Pathogen Interactions: VIII. Isolation of a Pathogen-synthesized Fraction Rich in Glucan That Elicits a Defense Response in the Pathogen's Host 1
A polysaccharide from the fungal pathogen Colletotrichum lindemuthianum causes browning and phytoalexin production when applied to the cut surfaces of bean (Phaseolus vulgaris) cotyledons and hypocotyls. The application of an amount of polysaccharide equivalent to less than 100 ng of glucose will elicit this response in the bean tissues. The polysaccharide h
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15. Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus
CapF, a capsular polysaccharide-assembling protein from S. aureus, has been crystallized using the hanging-drop vapour-diffusion method. Oprimization of the crystallization conditions by differential scanning calorimetry afforded a crystal of selenomethionine-substituted CapF that diffracted to a resolution of 2.80 Å.
International Union of Crystallography.
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16. Distinct Slime Polysaccharide Depolymerases of Bacteriophage-Infected Pseudomonas aeruginosa: Evidence of Close Association with the Structured Bacteriophage Particle
Five new polysaccharide depolymerases were isolated from cultures of Pseudomonas aeruginosa infected with phages 6, 7, 8, 9, and 10. The production of enzyme paralleled the release of phage. Depolymerase associated with phage 8 was active on slime polysaccharide A, whereas depolymerases associated with phages 6, 7, 9, and 10, like pseudomonas phage 2, hydrol
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17. THE ANTIGENIC COMPOSITION OF CRYPTOCOCCUS NEOFORMANS IV. : The Use of Paper Chromatography for Following Purification of the Capsular Polysaccharide1
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18. Purification and Properties of Colicin K
Colicin K was purified from Escherichia coli K-235 (Col K) as a single protein free from polysaccharide. Its molecular weight was estimated at 70,000.
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19. Extracellular Polysaccharides of Actinomyces viscosus
Polysaccharide(s) have been isolated from supernatants of broth cultures of Actinomyces viscosus, strain T6. The organism produces significant quantities (2 mg/mg of cell [dry weight], 108 mg/liter) of this polysaccharide in the absence of sucrose. Chemical analysis indicated that N-acetylglucosamine (62%) was the major component; galactose (7%), glucose (4%
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20. Capsular Polysaccharide of Clostridium perfringens Hobbs 10
A capsular polysaccharide was isolated from a strain of Clostridium perfringens Hobbs 10 type A by cold-water extraction of whole, heavily encapsulated cells. The water-soluble polymer was isolated by alcohol precipitation and purified by treatment with chloroform-butanol, cetytrimethylammonium bromide, and column gel permeation chromatography by using Bio-G
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21. Isolation of a coaggregation-inhibiting cell wall polysaccharide from Streptococcus sanguis H1.
Coaggregation between Streptococcus sanguis H1 and Capnocytophaga ochracea ATCC 33596 cells is mediated by a carbohydrate receptor on the former and an adhesin on the latter. Two methods were used to release the carbohydrate receptor from the gram-positive streptococcus, autoclaving and mutanolysin treatment. The polysaccharide released from the streptococca
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22. Chemical characterization and immunogenicity of capsular polysaccharide isolated from mucoid Staphylococcus aureus.
In this study we report the isolation and purification of the capsular polysaccharide elaborated by Staphylococcus aureus SA1 mucoid. The capsule was isolated from bacterial extracts and culture supernatants by a series of ethanol precipitations and enzyme digestions, followed by ion-exchange chromatography. Teichoic acid contamination was eliminated by oxid
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23. Partial Purification and Characterization of a Polysaccharide Depolymerase Associated with Phage-Infected Erwinia amylovora
Erwinia amylovora infected with bacteriophage ERA103 produced an enzyme which degraded the extracellular polysaccharide of noninfected cells. The depolymerase enzyme was purified 15-fold by a procedure which included ammonium sulfate precipitation, ultracentrifugation, CM-Sephadex batchwise separation, Sephadex G-50 column chromatography, and Sephacryl S-200
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24. BIOSYNTHESIS OF THE CHONDROITIN SULFATE-PROTEIN LINKAGE REGION: PURIFICATION AND PROPERTIES OF A GLUCURONOSYLTRANSFERASE FROM EMBRYONIC CHICK BRAIN*
The present paper describes the purification and properties of a glucuronosyltransferase isolated from 13-day embryonic chick brain. The enzyme catalyzes transfer of glucuronic acid from UDP-glucuronic acid to a series of low and high molecular weight compounds which contain terminal non-reducing β-D-galactose residues. Studies utilizing enzymatically degra