Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus

AUTOR(ES)

Miyafusa, Takamitsu

FONTE

International Union of Crystallography

RESUMO

CapF, a capsular polysaccharide-assembling protein from S. aureus, has been crystallized using the hanging-drop vapour-diffusion method. Oprimization of the crystallization conditions by differential scanning calorimetry afforded a crystal of selenomethionine-substituted CapF that diffracted to a resolution of 2.80 Å.

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