Polysaccharide Purification
Mostrando 1-12 de 86 artigos, teses e dissertações.
-
1. DEVELOPMENT OF A NEW PROCESS FOR PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM Streptococcus pneumoniae SEROTYPE 14
Abstract The main virulence factor of Streptococcus pneumoniae is the capsular polysaccharide (PS), which is the antigen of all current vaccines that are prepared with PS purified from serotypes prevalent in the population. In this work, three purification strategies were evaluated and a new process was developed for purification of serotype 14 PS (PS14), re
Braz. J. Chem. Eng.. Publicado em: 2016-09
-
2. Estudo dos modos batelada e reciclo externo estacionário para a separação do ácido hialurônico por cromatografia de exclusão por tamanho / Study of batch and steady state recycling modes for hyaluronic acid separation by size exclusion chromatography
The hyaluronic acid (HA) is a linear polysaccharide with important applications in the pharmaceutical, medical and cosmetics industry. Its purification is necessary because it is in a mixture coming from a variety of sources, animal and non-animal. The aim of this work is the separation of HA obtained by fermentation in synthetic medium, using the technique
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/09/2011
-
3. Alternative methods for purification of capsular polysaccharide produced by Haemophilus influenzae type b. / Métodos alternativos de purificação do polissacarídeo capsular de Haemophilus influenzae tipo b.
Haemophilus influenzae tipo b é uma bactéria Gram-negativa, patogênica causadora de meningites em crianças. A cápsula polissacarídica (PSb) é o principal fator de virulência e é usado como antígeno vacinal. O método clássico de purificação do PSb envolve várias etapas de precipitação com etanol, fenol e detergente catiônico (inflamável, co
Publicado em: 2009
-
4. Caracterização por ressonância magnética nuclear da celulose bacteriana acetilada em solução de dimetilacetamida/cloreto de lítio
Cellulose consists of an insoluble polysaccharide, used in industrial scale for manufacture of paper, films, or in the dust form, natural, hydrolyzed or derivatized. Bacterial cellulose, mainly produced by the bacterium Acetobacter xylinum, is identical to the vegetal one, but free of lignin and hemicellulose, wuich eliminates severe chemical processes of pu
Publicado em: 2008
-
5. ImobilizaÃÃo covalente de concanavalina a em goma de sementes de Parkia pendula magnetizada e sua aplicaÃÃo biotecnolÃgica
Protein immobilization in water insoluble supports is an important tool in biotechnology. Meanwhile, support magnetization increases its potential application since a magnetic field can be used to separate the support from the reaction system and its reuse afterwards. Among the proteins immobilized special attention have been given for lectins, mainly Concan
Publicado em: 2006
-
6. Cloning, expression and purification of proteins of surface, PsaA and fragments of PspA from Streptococcus pneumoniae / Clonagem, expressão e purificação das proteínas de superfície, PsaA e fragmentos de PspA de Streptococcus pneumoniae
Streptococcus pneumoniae is the main causative agent of bacterial pneumonia. The current vaccines available contain capsular polysaccharide conjugated or not with carrier proteins. However these are either too expensive or do not protect the high-risk groups. Surface proteins of S. pneumoniae, such as PsaA and PspA, are considered strong vaccine candidates.
Publicado em: 2005
-
7. Endo-beta-mananase de endosperma de Sesbania virgata (Cv.) Pers : purificação, caracterização e importancia na germinação e desenvolvimento da plantula
Many plant seeds have an endosperm, a tissue specialised either as storage or mechanical constraint to embryo growth. In many legume species, endosperms contain galactomannan. This polyssacharide is composed of a main chain of 1,4-β-mannan branched with variable amounts of 1,6-ά-linked galactosyl residues. Three enzymes produced by an aleurone laye
Publicado em: 2003
-
8. Purification of Polysaccharide Antigen from Leptospira biflexa Strain Urawa
Serologically active polysaccharide was isolated from the cells of Leptospira biflexa strain Urawa and purified. The constituents of this polysaccharide were characterized, and its serological specificity was partially examined.
-
9. Production of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography
We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal se
American Society for Microbiology.
-
10. Purification and immunochemical properties of Escherichia coli B polysaccharide cross-reacting with Salmonella typhi Vi antigen: preliminary evidence for cross-reaction of the polysaccharide with Escherichia coli K1 antigen.
An acidic polysaccharide of Escherichia coli B was isolated by a mild procedure and purified to homogeneity. The polysaccharide was found to react in Salmonella typhi Vi antisera and E. coli K1 antisera. Serological analysis and preliminary chemical characterization of the polysaccharide indicated that it is an aminouronic acid polymer which, although not st
-
11. Isolation and purification of anaphylactically active polysaccharide from human tubercle bacilli.
-
12. Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide.
Clinical isolates of Staphylococcus aureus have been previously classified into eight types on the basis of their capsular polysaccharide. The high prevalence of the type 8 capsular polysaccharide among bacteremic isolates suggests the importance of this capsular antigen in staphylococcal disease. The capsular polysaccharide was purified from extracts of thr