Polyphosphate
Mostrando 25-36 de 240 artigos, teses e dissertações.
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25. Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell.
The genes involved in polyphosphate metabolism in Escherichia coli were cloned behind different inducible promoters on separate plasmids. The gene coding for polyphosphate kinase (PPK), the enzyme responsible for polyphosphate synthesis, was placed behind the Ptac promoter. Polyphosphatase, a polyphosphate depolymerase, was similarly expressed by using the a
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26. ACCUMULATION OF INORGANIC POLYPHOSPHATE IN AEROBACTER AEROGENES I. : Relationship to Growth and Nucleic Acid Synthesis
Harold, F. M. (National Jewish Hospital, Denver, Colo.). Accumulation of inorganic polyphosphate in Aerobacter aerogenes. I. Relationship to growth and nucleic acid synthesis. J. Bacteriol. 86:216–221. 1963.—Growing cells of Aerobacter aerogenes contain traces of inorganic polyphosphate, but large amounts often accumulate when growth ceases as the result
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27. DEPLETION AND REPLENISHMENT OF THE INORGANIC POLYPHOSPHATE POOL IN NEUROSPORA CRASSA1
Harold, F. M. (National Jewish Hospital, Denver, Colo.). Depletion and replenishment of the inorganic polyphosphate pool in Neurospora crassa. J. Bacteriol. 83:1047–1057. 1962.—Turnover of the inorganic polyphosphate pool of Neurospora crassa was demonstrated in both growing and nongrowing mycelium. In nitrogen-deficient cultures, polyphosphate synthesis
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28. In situ 31P nuclear magnetic resonance for observation of polyphosphate and catabolite responses of chemostat-cultivated Saccharomyces cerevisiae after alkalinization.
The proposed pH buffering and phosphagenic functions of polyphosphate were investigated by subjecting chemostat-cultivated Saccharomyces cerevisiae to alkalinization (NaOH addition) and anaerobiosis. The subsequent changes in intracellular phosphate-containing species were observed in situ by nuclear magnetic resonance (NMR) spectroscopy by using the NMR cul
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29. Genetic manipulation of polyphosphate metabolism affects cadmium tolerance in Escherichia coli.
The polyphosphate metabolic pathways in Escherichia coli were genetically manipulated to test the effect of polyphosphate on tolerance to cadmium. A polyphosphate kinase (ppk) and polyphosphatase (ppx) mutant strain produced no polyphosphate, whereas the same strain carrying multiple copies of ppk on a high-copy plasmid produced significant quantities. The d
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30. Polyphosphate-degrading enzymes in Acinetobacter spp. and activated sludge.
Polyphosphate-degrading enzymes were studied in Acinetobacter spp. and activated sludge. Polyphosphate: AMP phosphotransferase activity in Acinetobacter strain 210A decreased with increasing growth rates. The activity of this enzyme in cell extracts of Acinetobacter strain 210A was maximal at a pH of 8.5 and a temperature of 40 degrees C and was stimulated b
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31. Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans
Polyphosphate accumulation by Paracoccus denitrificans was examined under aerobic, anoxic, and anaerobic conditions. Polyphosphate synthesis by this denitrifier took place with either oxygen or nitrate as the electron acceptor and in the presence of an external carbon source. Cells were capable of poly-β-hydroxybutyrate (PHB) synthesis, but no polyphosphate
American Society for Microbiology.
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32. Uranyl Precipitation by Pseudomonas aeruginosa via Controlled Polyphosphate Metabolism
The polyphosphate kinase gene from Pseudomonas aeruginosa was overexpressed in its native host, resulting in the accumulation of 100 times the polyphosphate seen with control strains. Degradation of this polyphosphate was induced by carbon starvation conditions, resulting in phosphate release into the medium. The mechanism of polyphosphate degradation is not
American Society for Microbiology.
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33. Cloning and Characterization of Polyphosphate Kinase and Exopolyphosphatase Genes from Pseudomonas aeruginosa 8830
Pseudomonas aeruginosa accumulates polyphosphates in response to nutrient limitations. To elucidate the function of polyphosphate in this microorganism, we have investigated polyphosphate metabolism by isolating from P. aeruginosa 8830 the genes encoding polyphosphate kinase (PPK) and exopolyphosphatase (PPX), which are involved in polyphosphate synthesis an
American Society for Microbiology.
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34. Polyphosphate and Orthophosphate Content of Nitrosomonas europaea as a Function of Growth
After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 μmoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 μmoles/g. As growth proceeded, the orthophosphate content decreased rapidly to a level of
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35. Properties of polyphosphate: AMP phosphotransferase of Acinetobacter strain 210A.
Polyphosphate:AMP phosphotransferase, an enzyme which catalyzes the phosphorylation of AMP to ADP at the expense of polyphosphate, was purified more than 1,500-fold from Acinetobacter strain 210A by streptomycin sulfate precipitation and by Mono-Q, Phenyl Superose, and Superose column chromatography. Streptomycin sulfate precipitation appeared to be an effec
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36. Basic amino acids and inorganic polyphosphates in Neurospora crassa: independent regulation of vacuolar pools.
At least 78%, and perhaps all, of inorganic polyphosphate is shown to be contained within the vesicles (vacuoles) of Neurospora crassa, where over 97% of the soluble arginine, lysine, and ornithine pools are known to accumulate. Furthermore, synthetic polyphosphate can concentrate arginine up to 400-fold from dilute (0.01 mM) solutions in equilibrium dialysi