DEPLETION AND REPLENISHMENT OF THE INORGANIC POLYPHOSPHATE POOL IN NEUROSPORA CRASSA1
AUTOR(ES)
Harold, F. M.
RESUMO
Harold, F. M. (National Jewish Hospital, Denver, Colo.). Depletion and replenishment of the inorganic polyphosphate pool in Neurospora crassa. J. Bacteriol. 83:1047–1057. 1962.—Turnover of the inorganic polyphosphate pool of Neurospora crassa was demonstrated in both growing and nongrowing mycelium. In nitrogen-deficient cultures, polyphosphate synthesis and degradation were in balance and no net changes occurred. When mycelium was suspended in a growth medium deficient in phosphate, polyphosphate was degraded to acid-soluble compounds, including adenosine triphosphate (ATP) and orthophosphate; net synthesis of nucleic acids and phospholipids occurred at the expense of polyphosphate. Various attempts were made to demonstrate direct formation of ATP from polyphosphate when oxidative and fermentative ATP generation were blocked. No evidence for this reaction could be obtained, suggesting that the primary product of polyphosphate degradation is not ATP but probably orthophosphate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279407Documentos Relacionados
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