Plant Protoplasts
Mostrando 1-12 de 364 artigos, teses e dissertações.
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1. Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.
Resumo Protoplastos in vivo do mesofilo foliar da cultivar de macieira “Anna” foram isolados de folhas com 15 dias de idade através de plasmólise em meios contendo manitol a 90 g L-1, por meia hora e em seguida a 130 g L-1, pelo mesmo tempo. Anteriormente ao isolamento dos protoplastos foi realizado um pré tratamento com mistura enzimática contendo
Rev. Bras. Frutic.. Publicado em: 05/08/2019
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2. A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis
Abstract Chloroplast development and chlorophyll (Chl) biosynthesis in plants are regulated by many genes, but the underlying molecular mechanisms remain largely elusive. We isolated a rice mutant named yss2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth. The mutant developed normal green leaves from
Genet. Mol. Biol.. Publicado em: 31/08/2017
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3. Transformação de plantas.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain
Planaltina. Publicado em: 2011
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4. A Preliminary Evaluation of Cell and Tissue Culture Methods Suitable for Screening Anthrocnose Disease Reactions in Tropical Yams.
Effect of different temperatures (15, 19, 25 and 29 °C), media type (PDA and Czapek Dox agar) and light on sporulation of a Caribbean pathovar of Colletotrichum gloe-osporioides f.sp. dioscoreae were studied. Czapek Dox agar and temperature of 25 °C were found to be best conditions for supporting growth and sporulation of this organism. Long-term fungal cu
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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5. Hibridação somática visando à produção de genitores tetraplóides para o melhoramento de cultivares copa de citros / Somatic hybridization in order to obtain tetraploid parents for citrus scion improvement
A hibridação somática via fusão de protoplastos é um método de combinação genética que agrega integralmente os dois genomas genitores, assim como suas respectivas organelas citoplasmáticas, sendo uma ferramenta alternativa aos métodos convencionais de melhoramento. O objetivo deste trabalho foi a obtenção de híbridos somáticos interespecífico
Publicado em: 2011
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6. Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.)
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively
Brazilian Journal of Microbiology. Publicado em: 2010-03
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7. Estresse osmótico e do retículo endoplasmático induzem morte celular programada de maneira dependente das proteínas NRPs / Osmotic- and ER-stress induce NRPs-dependent programed cell death
The identification of cell signaling pathways in response to different stresses and the interactions among these pathways have become major focus for understanding the molecular bases of plant cell-environment interactions. Recently, a novel integrative pathway between ER- and osmotic-stress has been described by our group. Among the target genes of the inte
Publicado em: 2007
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8. Protoplasts production from Napier grass and pearl millet triploid hybrids. 2007. 44 p. Dissertation (Master Program in Genetics and Plant Breeding) â Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil. / ObtenÃÃo de protoplastos de hÃbridos triplÃides entre o capim-elefante e milheto.
Buscando gerar maior variabilidade que resulte em avanÃos na obtenÃÃo de cultivares superiores os programas de melhoramento genÃtico do capim-elefante (2n = 4x = 28) realizam o cruzamento desta espÃcie com o milheto (2n = 2x = 14) Entretanto, o hÃbrido triplÃide (3x) resultante à infÃrtil impossibilitando sua utilizaÃÃo nesses A restauraÃÃo da f
Publicado em: 2007
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9. Etligera elatior (Jack) R. M. Smith: in vitro propagation, anatomy and obtation of protoplasts / Etligera elatior (Jack) R. M. Smith: propagaÃÃo in vitro, anatomia e obtenÃÃo de protoplastos
SILVA JÃNIOR, Jessà Marques. [Etligera elatior (Jack) R. M. Smith]: propagaÃÃo in vitro, anatomia e obtenÃÃo de protoplastos. 2007. 104p. DissertaÃÃo (Mestrado em Fisiologia Vegetal)- Universidade Federal de Lavras, Lavras, MG.* A floricultura tropical à uma atividade em ascensÃo no Brasil e no mundo, destacando-se como um agronegÃcio gerador de r
Publicado em: 2007
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10. Molecular and functional characterization of the NAC transfactor family from soybean (Glycine max) / Caracterização molecular e funcional de transfatores da família NAC de soja (Glycine max)
The identification of cell signaling pathways in response to different stresses has become a major focus for understanding the molecular bases of plantenvironment interactions. We have previously identified a set of soybean genes co-regulated by ER- and osmotic stresses, which possibly constitute a novel integrative pathway in plants. Among the identified ge
Publicado em: 2007
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11. Fusão de protoplastos visando a reconstrução da laranja azeda / Protoplast fusion aiming the reconstruction of sour orange
The aim of this work was to apply the technique of chemical fusion of protoplasts, in order to develop interspecific somatic hybrids between mandarins (Citrus reticulata) and pummelos (Citrus grandis), in order to produce similar to sour orange (Citrus aurantium). The sources for protoplasts were embryogenic suspension cultures of Pagetangelo (C. reticulata
Publicado em: 2006
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12. Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts
In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form were evaluated. Transformat
Genetics and Molecular Biology. Publicado em: 2002