Orc1 Cdc6
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Estudos sobre o componente ORC1/CDC6 da maquinaria de pré-replicação dos tripanossomas. / Studies of the Orc1/Cdc6 component of pre-replication machinery of trypanosomes.
Em eucariotos, a origem de replicação é reconhecida por um complexo ORC, a proteína Cdc6 e outras proteínas. Nos tripanossomas, encontramos somente uma proteína similar a Orc1 e Cdc6, que chamaremos de Orc1/Cdc6. Nesta tese serão descritos os estudos realizados sobre Orc1/Cdc6 do Trypanosoma cruzi e do Trypanosoma brucei. As proteínas recombinantes d
Publicado em: 2010
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2. Molecular dynamics at Trypanosoma cruzi nucleus. / Dinâmica molecular no núcleo de Trypanosoma cruzi.
In Trypanosoma cruzi the replication sites are located at nuclear periphery. In order to analyse the dynamics of molecules involved in replication, Orc/Cdc6 was used as a marker of pre-replication machinery and PCNA as a marker of replication machinery during the cell cycle of epimastigote form. Both molecules presented two nuclear patterns: dispersed patter
Publicado em: 2008
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3. Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus
Archaeal cell division cycle protein 6 (Cdc6)/Origin Replication Complex subunit 1 (Orc1) proteins share sequence homology with eukaryotic DNA replication initiation factors but are also structurally similar to the bacterial initiator DnaA. To better understand whether Cdc6/Orc1 functions in an eukaryotic or bacterial-like manner, we have characterized the i
Oxford University Press.
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4. Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae.
A novel cell cycle gene was identified by a computer search for genes partly homologous to known CDC genes, CDC6 of Saccharomyces cerevisiae and CDC18 of Schizosaccharomyces pombe, using the nucleotide sequence data base for S. cerevisiae produced by the Yeast Sequencing Project. The protein sequence coded by the cloned gene was found to be identical to that
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5. Binding of cyclin-dependent kinases to ORC and Cdc6p regulates the chromosome replication cycle
Cdc6p and the origin recognition complex (ORC) are essential for assembly of a pre-replicative complex (preRC) at origins of replication, before the initiation of DNA synthesis. In the absence of Cdc6p, cells fail to initiate DNA replication and undergo a “reductional” mitosis, in which the unreplicated chromosomes are randomly segregated to the spi
The National Academy of Sciences.
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6. Human CDC6/Cdc18 Associates with Orc1 and Cyclin-cdk and Is Selectively Eliminated from the Nucleus at the Onset of S Phase
In a two-hybrid screen for proteins that interact with human PCNA, we identified and cloned a human protein (hCdc18) homologous to yeast CDC6/Cdc18 and human Orc1. Unlike yeast, in which the rapid and total destruction of CDC6/Cdc18 protein in S phase is a central feature of DNA replication, the total level of the human protein is unchanged throughout the ce
American Society for Microbiology.
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7. Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus
The eukaryotic pre-replication complex is assembled at replication origins in a reaction called licensing. Licensing involves the interactions of a variety of proteins including the origin recognition complex (ORC), Cdc6 and the Mcm2-7 helicase, homologues of which are also found in archaea. The euryarchaeote Archaeoglobus fulgidus encodes two genes with hom
Oxford University Press.
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8. ATP bound to the origin recognition complex is important for preRC formation
The origin recognition complex (ORC) binds origins of replication and directs the assembly of a higher order protein complex at these sites. ORC binds and hydrolyzes ATP in vitro. ATP binding to the largest subunit of ORC, Orc1p, stimulates specific binding to origin DNA; however, the function of ATP hydrolysis by ORC is unknown. To address the role of ATP h
The National Academy of Sciences.
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9. Localization of proteins bound to a replication origin of human DNA along the cell cycle
The proteins bound in vivo at the human lamin B2 DNA replication origin and their precise sites of binding were investigated along the cell cycle utilizing two novel procedures based on immunoprecipitation following UV irradiation with a pulsed laser light source. In G1, the pre-replicative complex contains CDC6, MCM3, ORC1 and ORC2 proteins; of these, the
Oxford University Press.
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10. Human replication protein Cdc6 is selectively cleaved by caspase 3 during apoptosis
In eukaryotes, the initiation of DNA replication involves the ordered assembly on chromatin of pre-replicative complexes (pre-RCs), including the origin recognition complex (ORC), Cdc6, Cdt1 and the minichromosome maintenance proteins (MCMs). In light of its indispensable role in the formation of pre-RCs, Cdc6 binding to chromatin represents a key step in th
Oxford University Press.
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11. Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex.
cdc18+ of Schizosaccharomyces pombe is a periodically expressed gene that is required for entry into S phase and for the coordination of S phase with mitosis. cdc18+ is related to the Saccharomyces cerevisiae gene CDC6, which has also been implicated in the control of DNA replication. We have identified a new Sch. pombe gene, orp1+, that encodes an 80-kDa pr
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12. Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila
The initiation of DNA synthesis is thought to occur at sites bound by a heteromeric origin recognition complex (ORC). Previously, we have shown that in Drosophila, the level of the large subunit, ORC1, is modulated during cell cycle progression and that changes in ORC1 concentration alter origin utilization during development. Here, we investigate the mechan
Oxford University Press.